摘要
目的:研究不同光质对灵芝菌丝体生长及抗氧化酶活性的影响,为灵芝菌丝体培养提供科学的理论依据。方法:采用新型半导体发光二极管(LED)光源设置不同光质处理,动态观测灵芝菌丝体生长、多糖含量及抗氧化酶活性变化。结果:蓝光处理及黑暗对照条件下灵芝菌丝体生长较快,黑暗对照条件下菌丝体形态纤细、菌落淡薄,蓝光、白光处理菌丝体粗壮、菌落浓密。白光处理菌丝体生物量积累最高,蓝光次之。绿光处理灵芝菌丝体POD酶活性波动变化较大,生长中后期显著高于其他处理。白光处理菌丝体CAT酶在生长过程中始终保持较高活性。生长前期黄光处理菌丝体SOD酶活性最高,后期黑暗对照SOD酶活性较高。前期绿光处理菌丝体多糖含量较高,生长后期蓝光处理菌丝体多糖含量显著高于其他处理。结论:光质影响灵芝菌丝体生长代谢,蓝光利于灵芝菌丝体生长代谢。
Objective： To study the effect of light quality on growth,antioxidant enzyme activities of Ganoderma lucidum mycelium.Method： G.lucidum mycelium was cultured under different light qualities by light emitting diodes（LED）.The growth G.lucidum mycelium was observed and antioxidant enzyme activities was determined in different growth periods.Result： Under the red LED,the blue LED and dark condition（CK）,the mycelium grew faster than that under other light qualities.The white LED resulted in a largest increase in the amount of the mycelium and always kept the activities of CAT high level.Major fluctuations of POD activities emerged under the green LED,while enhanced severely in the late phase.Under the yellow LED,the activities of SOD appeared high level.However,SOD activities on dark（CK）raised obviously in late period.At the late stage,the content of mycelium polysaccharides was significant higher than that under the blue LED.Conclusion： The light quality could influence the growth and metabolism of G.lucidum mycelium.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第18期2471-2474,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81173660)
关键词
灵芝
光质
发光二极管
生长
抗氧化酶活性
灵芝多糖
Ganoderma lucidum
light quality
LED
growth
antioxidant enzyme activities
Ganoderma lucidum polysaccharide
作者简介
王立华，硕士研究生，主要从事药用真菌生理生化研究
[通信作者]兰进，Tel／Fax：（010）62899723，E-mail：lanjin60@hotmail．com
