摘要
以8个菌株(3株革兰氏阳性菌、3株革兰氏阴性菌和2株真菌)作为指示菌,采用管碟法对分离自南海硇洲岛马粪海胆(Hemicentrotus pulcherrimus)中的106株细菌(含放线菌)进行抗菌活性筛选,并对其中抗菌活性较强的菌株进行了基于16S rRNA基因序列的系统发育分析和生物学特性研究。结果显示,具有抗菌活性的细菌59株,占受试菌株总数的55.7%。综合分析形态特征、生理生化特征和基于16S rRNA基因序列的系统发育分析数据,结果表明,10株具有较强抗菌活性的菌株分别属于Halobacillus属(JSM 076029、JSM 076017、JSM 076062),Bacillus属(JSM 076053、JSM 076009),Pon-tibacillus属(JSM 076056),Planococcus属(JSM 076050),Jeotgalibacillus属(JSM 076100),Salinicoccus属(JSM 076102)和Serratia属(JSM 076077)。
The antimicrobial activity of 106 marine bacteria, isolated from sea urchins （Hemicentrotus pulcherrimus） collected from the coastal area of the Naozhou island in the South China Sea, were screened using the cylinder-plate method on 8 indicator microorganisms, i.e. three Gram-positive bacteria （Bacillus subtilis, Sarcina lutea, Staphylococcus aureus）, three Gram-negative bacteria （Aerobacter aerogenes, Escherichia coli, Proteus vulgaris）, and two fungi （Asperillus niger, Candida albican）. Out of 106 test strains, 59 were positive in antimicrobial activity （55.7%）, in which 10 strains exhibited strong antimicrobial activity. These 10 strains were submitted for 16S rRNA gene amplification and phylogenetic analysis after morphological, biochemical, and physiological characterization. It was found that the I 0 strains were within the phyla Firmicutes and Pro- teobacteria, belong to the genera Halobacillus （JSM 076029, JSM 076017, JSM 076062）, Bacillus （JSM 076053, JSM 076009）, Pontibacillus （JSM 076056）, Planococcus （JSM 076050）, Jeotgalibacillus （JSM 076100）, Salinicoccus （JSM 076102）, and Serratia （JSM 076077）.
出处
《海洋科学》
CAS
CSCD
北大核心
2011年第8期25-31,共7页
Marine Sciences
基金
国家自然科学基金项目(30970007)
关键词
海胆相关细菌
抗菌活性
生物学特性
系统发育
Associated bacteria with sea urchin
antimicrobial activity
biological characteristics
phylogenetic analysis
作者简介
作者简介：黄苛（1983-），男（瑶族），湖南溆浦人，硕士，研究方向为微生物资源与生态，E-mail：hkll520@163．com；
陈义光，通信作者，电话：0743—8564416，E-mail：mchenjsu@yahoo.com．cn
