摘要
通过对不同盐环境连云港海底底泥和青海湖底泥样品构建细菌16S rRNA克隆文库,对2个环境样品中细菌群落的多样性、丰富度和优势度等进行了比较,并对其群落组成结构进行了分析.结果表明,连云港海底底泥文库的多样性指数Shannon diversity(H)达到3.53,青海湖底泥文库的Shannon diversity(H)达到3.05,这2个样品的细菌群落均具有很高的多样性.连云港海底底泥中细菌序列主要类群为:变形菌门(Proteobacteria,49.2%),拟杆菌门(Bacteroidetes,29.2%);而青海湖底泥中细菌序列主要类群为:拟杆菌门(Bacteroidetes,60.0%),厚壁菌门(Firmicutes,26.0%).这些细菌中存在一些耐盐和嗜盐菌,对工业生产和高盐废水处理具有重要参考意义.
The 16S rRNA clone libraries of two different saline environments the Lianyungang marine sediment and the Qinghai Lake sediment were constructed.The Shannon diversity index,Chao and ACE richness index and Simpson dominance index of the bacterial communities in the two samples was compared,and the analysis for the bacterial community structures of this two samples was conducted.The results showed that the Shannon diversity index of Lianyungang marine sediment achieved 3.53,and that of Qinghai Lake sediment achieved 3.05,it was concluded that the bacterial communities in the two samples were diverse.The main bacterial communities in Lianyungang marine sediment included Proteobacteria（49.2%）and Bacteroidetes（29.2%）,and Bacteroidetes（60.0%）and Firmicutes（26.0%）were the main bacterial communities in Qinghai Lake sediment.Some halotolerant and halophilic bacteria were found,which were important for industrial production and high saline wastewater treatment.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2011年第9期2681-2688,共8页
Environmental Science
基金
科技部国际科技合作项目(2006DFA91870)
高等学校博士学科点专项科研基金项目(20090095120018)
关键词
盐环境
16SrRNA克隆文库
细菌多样性
耐盐嗜盐菌
saline environment
16S rRNA clone library
bacterial diversity
halotolerant and halophilic bacteria
作者简介
侯梅锋（1985-），女，硕士研究生，主要研究方向为高盐微生物，E-mail：hou．meifeng@163．com
通讯联系人。E-mail：hslongcrees@163．com
