摘要
选用26个来自国内外具有不同毒性谱的条锈菌菌系,对50个甘肃省主要生产品种(系)及抗源材料进行苗期条锈病抗性鉴定,结合系谱分析,分析推导其所含抗条锈基因,同时对43个品种(系)进行了分子检测。推导分析结果表明,中梁25含有Yr3及未知抗病基因;兰天20含有Yr3a+Yr4a+Yr16及未知抗病基因;Y9220-12含有Yr9+YrCle及未知抗病基因;兰天14、陇原932、陇育216及陇原992含有Yr9及未知抗病基因;陇鉴9343、93保4-4、天选43、贵农22含有Yr10+YrMor;兰天19含有Yr12及未知抗病基因;兰天17、95-111-3、98-178-3-2-4、92R137含有Yr26。分子检测结果发现兰天21等14个品种(系)含有Yr9,兰天17、92R178含有Yr26。其余品种(系)含有未知抗病基因。田间抗性鉴定及监测结果显示,供试品种苗期抗条锈性和成株期抗条锈性结果不完全一致,兰天16等10个品种(系)可能具有成株抗性,兰天14等10个品种(系)可能具有慢条锈性。
By means of artificial inoculation of 26 Puccinia striiformis isolates with different spectra of pathogenicity at seedling stage, the resistance to strip rust was identified in 50 wheat varieties （lines） from Gansu province, China. In combination with pedigree analysis and resistance reactions of 30 standard varieties with known resistant genes, the resistance genes in the 50 varieties （lines） were postulated. The resistant genes Yr5, Yr10, Yr15, Yr26, and Yr9 （1B/1R） were validated using molecular markers in 43 varieties （lines）. The results of resistance gene postulation showed that Zhongliang 25 was carrying Yr3 and unknown genes. The vareity Lantian 20 was postulated to possess Yr3a, Yr4a, Yr16, and unknown genes. The line Y9220-12 was postulated to possess Yr9, YrCle, and unknown genes. The varieties （lines） Lantian 14, Longyuan 932, Longyu 216, and Longyuan 992 were postulated to possess Yr9 and unknown genes. Genes Yr10 and YrMor were postulated in Longjian 9343, 93-Bao 4-4, Tianxuan 43, and Guinong 22. The variety Lantian 19 was postulated to possess Yr12 and unknown resistant genes. Gene Yr26 was postulated in Lantian 17, 95-111-3, 98-178-3-2-4, and 92R137. Fourteen varieties （lines） were detected to be Yr9 （1B/1R） wheatrye translo-cation, which accounted for 32.6% of the total varieties. Yr26 was detected in 92R178 and Lantian 17. Other varieties （lines） were controlled by unknown genes. Although most varieties （lines） tested were highly susceptible at seedling stage, some of them showed adult resistance in the observation pool in Longnan, Gansu, China. Ten varieties （lines） including Lantian 10 might possess adult resistance and another 10 varieties （lines） including Lantian 14 might be varieties with slow rusting characteristics.
出处
《作物学报》
CAS
CSCD
北大核心
2011年第8期1360-1371,共12页
Acta Agronomica Sinica
基金
国家公益性行业(农业)科研专项(200903035)
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室开放项目(SKL2011OP10)
甘肃省农牧厅农业生物技术与开发项目(GNSW-2007-16)资助
关键词
小麦条锈病
抗病基因
基因推导
分子检测
甘肃省
Wheat stripe rust； Resistance gene； Gene postulation； Molecular detection； Gansu province of China
作者简介
E-mail：caoshiqin6702@163．com，Tel：0931-7616458
通讯作者（Corresponding authors）：金社林，E－mail：jinshelin@163．com
通讯作者（Corresponding authors）：尚勋武，E－mail：shangxunwu@163．com
