摘要
采用高效毛细管电泳(HPCE)分离紫外检测方法,比较了乙腈萃取、乙腈萃取-固相萃取(SPE)、基质固相分散萃取(MSPD)和MSPD-SPE 4种方法对鸡组织样品中青霉素、氨苄青霉素、阿莫西林残留的前处理效果。结果表明,与其他方法相比,MSPD方法的回收率高、图谱背景干净。据此建立了鸡组织中青霉素、氨苄青霉素、阿莫西林残留的MSPD-HPCE/UV同时检测方法。在优化色谱条件下,青霉素和氨苄青霉素标准品的线性范围为100～1 000μg/L,阿莫西林为50～1 000μg/L;在50、500、1 000μg/kg的加标水平下,青霉素、氨苄青霉素、阿莫西林在鸡组织中的回收率为73%～96%,相对标准偏差(RSD)为2.9%～9.8%,方法的日内和日间RSD为4.0%～12.0%。青霉素、氨苄青霉素、阿莫西林的检出限分别为20、30、10μg/kg,定量下限分别为50、50、40μg/kg。
Different pretreatment methods（e.g.acetonitrile extraction,acetonitrile extraction-solid phase extraction（SPE）,matrix solid phase dispersion（MSPD） extraction and MSPD-SPE） for the simultaneous analysis of penicillin,ampicillin and amoxicillin in chicken tissues were compared with HPCE.The results indicated that,among these methods,the MSPD method was of high precision,high recovery,easy operation and good practicability.Under the optimal chromatographic conditions,the calibration curves were linear in the range of 100-1 000 μg/L for penicillin and ampicillin,and 50-1 000 μg/L for amoxicillin.At the spiked concentrations of 50,500,1 000 μg/kg,the recoveries of these three drugs in chicken tissues ranged from 73% to 96% with relative standard deviations（RSDs） of 2.9%-9.8%.The intra-RSDs and inter-RSDs ranged from 4.0% to 12.0%.The detection limits of penicillin,ampicillin and amoxicillin were 20,30,10 μg/kg,respectively,and their quantitation limits were 50,50,40 μg/kg,respectively.The method could meet the requirements for the residue analysis in accuracy and precision.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第8期892-896,共5页
Journal of Instrumental Analysis
基金
国家十一五科技支撑项目子项目(2006BAK02A08-4)
关键词
基质固相分散萃取
高效毛细管电泳
样品前处理
青霉素类药物
残留
鸡组织
matrix solid phase dispersion extraction
high performance capillary electrophoresis（HPCE）
sample pretreatment
penicillins
residues
chicken tissues
作者简介
通讯作者：汪雪雁,硕士,研究方向：动物源性食品安全,Tel： 13514980970, E-mail： wxy7OO303@ahau, edu. cn
