摘要
目的:通过研究缩泉丸对下丘脑-垂体-肾上腺皮质系统的调节作用,阐明其补肾缩尿的机制。方法:采用腺嘌呤(150 mg.kg-1,ig 4周)造成肾虚多尿大鼠模型。造模第2周起开始按组ig缩泉丸(1.170,0.585,0.293 g.kg-1)、金锁固精丸(0.968 g.kg-1)连续4周,另设正常对照组、模型对照组。利用ELISA法、放射免疫法检测血浆促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、环磷酸腺苷(cAMP)的含量,并取垂体、肾上腺称质量计算脏器系数。结果:与模型组比较,缩泉丸可显著提高肾虚多尿大鼠血中CRH,ACTH,cAMP含量,并可提高其垂体、肾上腺系数。结论:缩泉丸可通过上调下丘脑-垂体-肾上腺皮质系统(HPA)系统的功能发挥调节人体水液代谢的作用,从调节内分泌系统角度阐明了缩泉丸补肾缩尿的作用机制。
Objective： To investigate the mechanism of nourishing kidney and reducing urine by investigating the regulative action of Suoquanwan to HPA.Method： The rat model of kidney deficiency and diuresis was established by adenine（150 mg·kg-1,ig 4 weeks）,From the second week after the model establishment,the treatments were ig given,including Suoquanwan（1.170,0.585,0.293 g·kg-1）,Jinsuo Gujin Wan（0.968 g·kg-1）.The contents of CRH,ACTH,cAMP in blood in model group,Suoquanwan group,Jinsuo Gujin Wan group and controll group were determined with ELISA and RIA technology,and also the hypophysis index and adrenal gland index were calculated.Result：Compared with the model group,Suoquanwan could remarkedly increase the contents of CRH,ACTH,cAMP in blood in rats with kidney deficiency and diuresis and improve the hypophysis exponent and adrenal gland exponent.Conclusion： Suoquanwan can enhance the action of body adjusting water metabolize by coordinating the function of HPA.The results of the experiment expound the mechanism of nourishing the kidney and reducing urine of Souquan Wan from the point of view of endocrine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第16期125-127,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30873425)
江苏省自然科学基金项目(2010GQY0186)
作者简介
[通讯作者]李淑雯，副教授，中药学博士，从事中药复方药理研究，Tel：15879846716，E-mail：lswzyh@qq．com
