摘要
利用富立叶变换红外光谱对水溶性牛血清白蛋白(BSA)羟基磷灰石复合物的组成及结构进行了研究.结果表明,其组成具有非化学计量的性质,且复合物中BSA与羟基磷灰石间存在相互作用.从而增加了羟基磷灰石在水中的溶解度.正是由于羟基磷灰石与蛋白质形成了水溶性的复合物,使羟基磷灰石在蛋白质结构的基质上成核和自组装成为可能,从而引起和促进了生物矿化过程.
Calcium hydroxyapatite [Ca 10 (OH) 2(PO 4) 6]and protein are main compositions of bones and tooth of animals. The interaction of protein with calcium hydroxyapatite plays an important role in biomineralization process. In this paper, a series of soluble bovine serum albumin (BSA) calcium hydroxyapatite complexes in aqueous solution produced by different initial mole ratio of solid calcium hydroxyapatite and BSA were studied using FTIR spectroscopy. The results show that the composition of the soluble BSA calcium hydroxyapatite complexes is nonstoichiometric. Calcium hydroxyapatite may be involved in hydrogen bonding to and coordinating with BSA in the soluble complexes.The interaction of BSA with calcium hydroxyapatite increased the solubility of calcium hydroxyapatite in aqueous solution, which is helpful for the formation of self organized structure of protein calcium hydroxyapatite complexes.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1999年第12期1064-1069,共6页
Acta Physico-Chimica Sinica
基金
国家自然科学基金! (2 94 710 0 2 )
重点基金
国家教委博士点基金! (0 94 0 119)
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关键词
牛血清白蛋白
FTIR
羟基磷灰石
FT-IR光谱
Bovine serum albumin, Calcium hydroxyapatite, FT IR spectroscopy, Interaction, Biomineralization
