摘要
目的探讨B细胞特异的莫洛尼白血病毒插入位点1(BMI-1)基因在子宫颈癌组织中的表达及其与临床病理参数的关系,初步评价BMI-1基因在子宫颈癌发生、发展中的作用及其意义。方法收集30例子宫颈癌患者的癌组织及10例子宫肌瘤患者的子宫颈组织,采用实时定量PCR方法检测患者子宫颈组织的BMI-1 mRNA的表达情况;采用SP免疫组织化学法对以上40例手术标本石蜡切片进行染色,检测BMI-1蛋白的表达。结果子宫颈癌组织中BMI-1 mRNA及蛋白的表达高于正常子宫颈组织,差异具有统计学意义(P<0.05);中低分化子宫颈癌组织中BMI-1蛋白的表达高于高分化子宫颈癌组织中的表达,差异具有统计学意义(P<0.05);伴有淋巴结转移的子宫颈癌组织中BMI-1蛋白的表达高于无淋巴结转移的子宫颈癌组织,差异具有统计学意义(P<0.05);BMI-1蛋白在子宫颈鳞癌与腺癌的相对表达量差异无统计学意义(P>0.05)。结论 BMI-1基因的过表达在子宫颈癌的发生发展中有重要意义,与子宫颈癌组织的分化程度、淋巴结转移密切相关。
Objective To investigate the expression of B cell-specific MLV insertion site-1（BMI-1） and its clinicopathologic significance in cervical cancer.Methods The expression of BMI-1 mRNA was detected by real time polymerase chain reaction（PCR） in 30 cervical cancer samples and 10 normal cervical samples.The expression of BMI-1 protein was examined by SP immunohistochemistry in archival paraffin-embedded tissue specimens.Statistical analysis was performed to investigate the association between the clinicopathologic features and BMI-1 expression.Results Real time-PCR demonstrated that the BMI-1 mRNA levels in cervical cancer samples were significantly higher than those in normal cervical samples（P 〈 0.05）.Immunohistochemical staining showed that BMI-1 protein was highly expressed in cervical cancer tissues than normal cervical tissue（P 〈 0.05）.Expression of BMI-1 protein was highly correlated with the differentiation degree of tumor and lymph node metastasis（P 〈 0.05）.No significant difference of BMI-1 protein expression was found between cervical squamous cell carcinoma and adenocarcinoma（P 〉 0.05）.Conclusion Overexpression of BMI-1 plays an important role in the oncogenesis and development of cervical cancer.Expression of BMI-1 protein was highly correlated with the differentiation degree of tumor and lymph node metastasis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第8期719-721,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30973191)
辽宁省科学技术计划(2008225004)
盛京自由研究者计划(200806)
作者简介
高翔（1983-），女，医师，硕士研究生．
张淑兰，E—mail：zs10909@sina.com
