摘要
该研究建立了高效液相色谱法定量测定烟丝及烟气中的单糖、双糖,确定的色谱条件如下:Waters NH2氨基色谱柱(250 mm×4.6 mm),乙腈︰水的体积比85︰15为流动相,流速1.0 mL/min,示差折光检测器,40℃柱温,进样量10μL,外标法定量。该方法的精密度RSD<5%,回收率大于96%。在此色谱条件下20 min内完成了单糖(葡萄糖、果糖、半乳糖)、双糖(蔗糖、麦芽糖)的分离,结果比较理想。研究表明此新建方法准确而快速,并能适用于烟丝及烟气中单糖、双糖的测定。
The method of HPLC was developed for determining the carbohydrates and monosaccharides of tobacco and smoke by using Waters HPLC with 250 mm×4.6 mm NH2 column and dual detectors.The mobile phase was composed of acetonitrile and water（85∶15）,at a flow rate of 1.0 mL/min.The added quantity is 6 mL.The temperature of the column was 40 ℃.The method was quantified by an external standard mode with lower RSD than 5% and greater recovery than 96%.Under these conditions,the arbohydrates and monosaccharides were determined in 20 min with satisfactory results.This new method is rapid,simple and accurate,and useful for determining the carbohydrates and monosaccharides.
出处
《食品工业》
北大核心
2011年第7期95-97,共3页
The Food Industry
基金
云南中烟公司项目"中试卷烟清香型风格品类特征的研究"(2009JC05)
关键词
高效液相色谱法
烟丝
烟气
水溶性糖
high performance liquid chromatography
tobacco
smoke
water soluble sugars
