摘要
目的探讨p53基因突变在肺癌中对TSG101/MDM2信号通路影响的临床病理学意义。方法采用免疫组织化学方法检测185例肺癌组织标本中TSG101、MDM2及p53的表达,用聚合酶链反应单链构象多态性(Polymerase Chain Reaction,single-strand conformation polymorphism,PCR-SSCP)分析法检测p53突变情况,以及Western blot检测TSG101在肺癌组织和正常肺组织中的表达。结果 (1)肺癌组中p53蛋白的总阳性率为80.54%(149/185),PCR-SSCP检测结果显示总突变率为56.67%(17/30),p53蛋白表达与病理分期、淋巴结转移有相关性(P<0.05);TSG101蛋白的低表达率为58.92%(109/185),Western blot结果显示TSG101在癌组织中的表达明显低于对照组(P<0.05),TSG101蛋白表达与病理分期、分化、淋巴结转移有相关性(P<0.05);MDM2蛋白的过表达率为77.84%(144/185),MDM2蛋白表达与病理分期、淋巴结转移有相关性(P<0.05)。(2)在p53阳性的149例中TSG101阳性76例,MDM2阳性139例,在p53阴性的36例中TSG101阳性33例,MDM2阳性5例。p53与TSG101两者共表达率为41.08%,一致性为42.70%。p53与MDM2两者共表达率为75.14%,一致性为91.89%。结论 (1)p53/MDM2上调与TSG101表达下调肺癌的发生及生物学行为有关。(2)当p53突变时,TSG101与MDM2的表达呈负相关关系。
Objective To explore the clinical pathology effect of gene mutation of p53 in lung cancer on TSG101/MDM2 signal passway.Methods Immunohistochemical method was adopted to detect the expression of TSG101,MDM2 and p53 in lung cancer tissues of 185 cases.PCR-SSCP was used to detect the gene mutation of p53.Western blot method was adopted to detect the expression of TSG101 in tumor tissues and normal tissues.Results（1）The overall positive rate of p53 protein in the group of cancer was 80.54%（149/185）.The result of PCR-SSCP showed that the overall mutation rate was 56.67%（17/30）.The expression of p53 protein had correlation with the stages of pathology and the lymph node（P0.05）.The low expression rate of TSG101 protein was 58.92%（109/185）.The result of Western blot showed that the expression of TSG101 in the group of cancer tissues was significantly lower than that in the control group（P0.05）.The expression of TSG101 protein had correlation with the stages of pathology,differentiation and lymph node metastasis（P0.05）.The excess expression rate of MDM2 protein was 77.84%（144/185）.The expression of MDM2 protein had correlation with the stages of surgery pathology and lymph node metastasis（P0.05）.（2）There were 76 cases of TSG101 positive and 139 cases of MDM2 positive in the 149 cases p53 positive,respectively.There were 3 cases of TSG101 negative and 31 cases of MDM2 negative in the 36 cases of p53 negative,respectively.The altogether expression rate was 91.89%.Conclusion（1）The up-regulated expression of p53/MDM2 and decline of TSG101 have correlation with the occurrence of lung cancer and its biology behavior.（2） When p53 gene is mutation,the expression of TSG101 and MDM2 is negative correlation.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第7期774-777,共4页
Cancer Research on Prevention and Treatment
基金
河南省教育厅重点科研课题资助项目(2006310024)
新乡市科技局重点科研课题资助项目(07S050)
作者简介
作者简介：杨廷桐（1953-），男，硕士，教授，主要从事肺癌发生与阻断的研究
