摘要
目的 研究立克次体的１６ＳｒＲＮＡ基因序列的差异，为其分类和鉴定提供依据。方法 从基因数据库中选择不同的立克次体属代表种的１６ＳｒＲＮＡ基因序列，在计算机上用多序列排列程序对序列进行同源位排列和对比分析，以及依据序列的相似程度构建进化树。结果 立克次体的１６ＳｒＲＮＡ基因含有４ 个高变区，这些高变区的序列具有种或属的特异性，依据序列的相似程度构成的进化树可将立克次体目内成员分为柯克斯体、巴通体、立克次体、埃立克体等４ 个大基因群，每个大基因群含有若干个小基因群。结论 １６ＳｒＲＮＡ基因高变区的序列可用于设计立克次体种、属特异性ＰＣＲ引物和杂交探针；１６ＳｒＲＮＡ
Aim\ To find the sequences differences of the rickettsial 16S rRNA genes in order to identify the rickettsial organisms.Methods\ The 16S rRNA sequences of the represent species of rickettsial organisms were obtained from GenBank and they were arranged and compared based on the homologous base positions of the sequenes by a multiple alignment program in computer,the evolution tree of the 16S rRNA genes was established depended on their sequence similarities.Results\ Four high divergent regions were identified among the 16S rRNA sequences of the rickettsial organisms and the sequences in these regions were species/genus specific;the rickettsial organisms were divided into four large groups(Coxiella,Bartonalla,Rickettsia,and Ehrlichia)in the evolution tree of the 16S rRNA genes.Conclusion\ The sequences of the high divergent regions of the rickettsial 16S rRNA genes may be useful to design species/genus specific primers or probes for the identification of the rickettsial organisms;based on the 16S rRNA genes analysis,the classification and identification of rickettsial organisms are reliable.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第6期18-20,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金
