摘要
试验旨在研究黄芪多糖(astragalus polysaccharide,APS)注射液作为猪瘟疫苗稀释液及黄芪多糖作为疫苗佐剂提供数据支持。用1%的黄芪多糖注射液、猪瘟疫苗专用稀释液、生理盐水3种不同猪瘟疫苗稀释液稀释兔源猪耐热保护剂活疫苗免疫兔体,比较免疫后不同时间段内血液中的淋巴细胞的活性及血清中的猪瘟抗体、白介素2(interleukin 2,IL-2)、白介素6(interleukin 6,IL-6)、γ干扰素(interferonγ,IFN-γ)、新蝶呤(neopterin,Npt)水平,综合评价3种不同猪瘟疫苗稀释液对兔源猪瘟耐热保护剂活疫苗在兔体内免疫效果的影响。结果表明,黄芪多糖能不同程度的提高抗体水平和多种细胞因子的含量。因此,黄芪多糖注射液可以作为猪瘟疫苗稀释液或改良现有猪瘟疫苗稀释液,黄芪多糖也可以作为猪瘟疫苗佐剂。
To provide the basis of APS used for swine fever vaccine diluent and vaccine adjuvant.The rabbits in three groups were intramuscular injected with activated thermostabilized vaccine of swine fever with diluent of 1% astragalus polysaccharide（astragalus polysaccharide,APS） injection,dedicated diluent for swine fever vaccine and physiological saline respectively.After vaccination,the activty of lymphocyte,antibody of swine fever in serum and contents of interleukin 2,interleukin 6,interferon γ and neopterin were determined to evaluate the impact of these three diluents on vaccination effects to swine fever vaccine.The results showed that APS raised contents of antibody of swine fever in serum and some cell factors.APS could be used for swine fever vaccine diluent or improved diluent and swine fever vaccine adjuvant.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第7期66-70,共5页
China Animal Husbandry & Veterinary Medicine
关键词
黄芪多糖
淋巴细胞活性
抗体
白介素
干扰素
新蝶呤
astragalus polysaccharide
activity of lymphocytes
antibody
interleukin
interferon
Neopterin
作者简介
朱辉（1983-），男，山东人，硕士生，研究方向：临床免疫。
通信作者：沈志强，研究员，博士生导师，研究方向：兽医生物制品研制。Email：bzshenzq@163．com
王振勇，副教授，硕士生导师，研究方向：动物营养代谢病。Email：wzy@sdau．edu．cn
