摘要
目的:研究结肠癌转移相关基因1(metastasis-associated in colon cancer1,MACC1)的表达对结肠癌肝转移的影响。方法:将融合绿色荧光蛋白(green fluorescent proteins,GFP)的慢病毒表达载体分别感染具有不同转移潜能的人结肠癌细胞株SW1116和HCT116。给予BALB/c-nu/nu裸鼠脾脏注射感染后的结肠癌细胞,构建结肠癌肝转移模型。注射后35d,在荧光解剖显微镜下观察肝脏内结肠癌细胞的转移情况,计算肝转移率。应用蛋白质印迹法检测SW1116和HCT116细胞中MACC1蛋白的表达水平,评估MACC1的表达水平与肝转移率的关系。结果:SW1116细胞的肝转移率明显高于HCT116细胞。MACC1蛋白在HCT116细胞中呈低表达,而在SW1116细胞中呈高表达,差异有统计学意义(P<0.01)。结论:MACC1蛋白的表达水平可能与结肠癌的肝转移能力呈正相关。
Objective： To investigate the effect of expression of metastasis-associated in colon cancer 1 （MACC1） on the liver metastasis from colon cancer. Methods： The SWl 116 and HCT116 cells, which had different liver metastasis abilities, were infected with lentiviral vector pCDH cDNA/ green fluorescent protein （GFP） used as a tracer. The liver metastasis model in BALB/c-nu/nu nude mice was established by spleen injection of these two infected colon cancer cell lines. The liver metastasis from colon cancer cells was observed under a fluorescence dissecting microscope, and the liver metastasis rate was calculated. The expression levels of MACC1 protein in SWl 116 and HCT116 cells were detected by Western blotting, and the correlation between the MACC1 protein expression and the liver metastasis rate was evaluated. Results： The liver metastasis rate of SW1116 cells was higher than that of HCT116 cells. The MACC1 protein expression in SWl 116 cells was high, but it was low in HCT116 cells, and the difference between the two colon cancer cells was significant （P〈0.01）. Conclusion： The expression level of MACC1 may have a positive association with the liver metastatic ability of colon cancer cells.
出处
《肿瘤》
CAS
CSCD
北大核心
2011年第6期513-516,共4页
Tumor
作者简介
Correspondence to：WANG Zheng（王争）E—mail：wangzh1972@126．com
