摘要
目的:研究山豆根致大鼠肝脏损害的毒性机制。方法:SD大鼠,随机分为山豆根组和正常组,山豆根组灌服10g.kg-1山豆根水煎液7 d后,灌服20 g.kg-1山豆根水煎液至26 d,正常组灌服等容量蒸馏水,连续给药26 d后,25%乌拉坦腹腔麻醉,腹主动脉取血,离心,取血清测ALT,AST。摘取肝脏,加冷生理盐水制成10%肝匀浆,离心,取上清液,测定肝组织中SOD,GSH活性,MDA,γ-GT蛋白含量;采用双抗体夹心ABC-ELISA法测定大鼠肝组织TNF-α含量;采用免疫组织化学SABC法测定大鼠肝组织ICAM-1蛋白表达量。结果:与正常组比较,口服山豆根水煎液后,大鼠血清ALT,AST值显著升高(P<0.01);SOD/MDA,GSH显著下降(P<0.01);γ-GT值显著上升(P<0.05);TNF-α含量升高(P<0.05);ICAM-1阳性表达明显增强(P<0.01)。结论:大鼠灌胃给予山豆根后,可对肝脏产生明显的毒性,其机制可能与自由基及炎症因子的产生有关。
Objective： To study the hepatotoxicity mechanism of rats that induced by Sophorae Tonkinensis Radix et Rhizoma.Method： The SD rats were randomly divided into the control and Sophorae Tonkinensis Radix et Rhizoma（RRST） group,given distilled water and RRST 10 g·kg-1 separately by orally for 7 days,and RRST 20 g·kg-1 for other days until the 26th day.Blood was drawn from abdominal aorta after the rats were anesthetized by 25% urethane,and then centrifugally processed to get the serum for detection of ALT and AST.The liver tissues of control and experimental group were taken to prepare liver homogenate with cold NS,and centrifugally processed to get the supernatant.The activities of SOD and GSH,the content of γ-GT and MDA were detected according to the methods of kit.The tumor necosis factor（TNF-α）was detected by ABC-ELISA.The expression of ICAM-1 was detected by immunohistochemistry.Result： After the rats were given RRST by oral for 26 days,the ALT and AST activities in serum increased,the content of GSH and the ratio of SOD and MDA decreased,the content of γ-GT and TNF-α,the masculine expression of ICAM-1 increased.Conclusion： After the rats were given RRST,the liver can be damaged obviously,and the mechanism of hepatotoxicity perhaps related to free radical and inflammatory factor.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第13期1821-1823,共3页
China Journal of Chinese Materia Medica
基金
国家重点基础研究发展计划(973)项目(2009CB522807)
国家"重大新药创制"科技重大专项(2009ZX09502-002)
关键词
山豆根
大鼠
肝毒性
Sophorae Tonkinensis Radix et Rhizoma
rat
hepatotoxicity
mechanism
作者简介
[作者简介]李峰杰，在读硕士，Tel：（021）51323053，E—mail：lfj1210@126．com
[通信作者】姚广涛，Tel：（021）51322472，E—mail：ygt1969@yahoo．com．cn
