摘要
目的建立测定灵芝子实体中灵芝酸A含量的反相高效液相色谱法（RP-HPLC）测定方法。方法采用Promosil-C18（250 mm×4.6 mm,5μm）色谱柱,以乙腈-体积分数0.03%甲酸水溶液为流动相梯度洗脱,流速为0.8 ml/min,检测波长254 nm。结果灵芝酸A的质量浓度在1.1~11μg范围内与峰面积呈良好线性关系,r=0.999,Y=1.121X-0.112,RSD=1.61%（n=6）。结论 RP-HPLC测定方法简便、快速、准确、重复性好,可用于控制灵芝子实体的质量。
Objective To establish RP-HPLC method of determining the content of Ganoderma lucidum acid A in its fruiting body.Methods We adopted Promosil-C18（250 mm×4.6 mm,5 μm） column and used acetonitrile-0.03% volume fraction of formic acid as the mobile phase gradient elution with flow rate 0.8 ml/minand detection wavelength 254 nm.Results The concentration range of Ganoderma lucidum acid A was in 1.1-11 μg and the peak area showed good linearity,r=0.999,Y= 1.121X-0.112,RSD= 1.61%（n=6）.Conclusion The method is simple,rapid,accurate,repeated,and can be used to control the quality of Ganoderma lucidum fruiting bodies.
出处
《福建医药杂志》
CAS
2011年第3期56-58,共3页
Fujian Medical Journal
关键词
灵芝子实体
灵芝酸A
反相高效液相色谱法
含量测定
Ganoderma lucidum fruiting body
Ganoderma lucidum acid A
RP-HPLC
determination of content
