摘要
目的:研究原代培养的BALB/c小鼠大脑皮质小胶质细胞经β-淀粉样肽1-42(Aβ1-42)刺激后的形态学变化及细胞因子表达情况。方法:小胶质细胞用2次温和胰酶消化法分离,经预处理的Aβ1-42刺激后,相差显微镜观察形态学改变,RT-PCR检测IL-1β、-6基因表达水平。结果:小胶质细胞经Aβ1-42刺激后6 h细胞形态学发生明显改变;刺激24 h后IL-1β、-6基因表达水平明显上调(P<0.05)。结论:Aβ1-42可明显活化小胶质细胞,并诱导炎性细胞因子的表达。
Objective：To investigate the effect of β-amyloid 1-42（Aβ1-42）on the morphology and cytokine production of primary cultured BALB/c mice cortical microglia.Methods：The microglia were isolated by two-step mild trypsin method.After treatment with Aβ1-42,the morphology of microglia was observed under phase-contrast microscopy,and the gene expressions of IL-1β,-6 were detected.Results：After treatment with Aβ1-42 for 6 hours,the microglia showed a typical activated morphology.After 24 hours,the gene expressions of IL-1β,-6 were significantly higher than control（P〈0.05）.Conclusion：The microglia can be activated and can express high level IL-1β,-6 after it is stimulated by Aβ1-42.
出处
《汕头大学医学院学报》
2011年第2期75-77,共3页
Journal of Shantou University Medical College
基金
广东省医学科研基金资助项目(A2010392)
国家自然科学基金资助项目(81001340
30771988)
汕头市重点科技计划资助项目(汕府科[2009]70号-200903)
作者简介
作者简介：辛岗（1973-），女，山西省太原市人，硕士，副教授，主要从事神经免疫学研究。
通信作者：李康生E-mail：ksli@stu．edu．cn
