摘要
从婴儿粪便中分离获得能够产生抑菌活性物质的乳酸菌,通过排除有机酸、过氧化氢的干扰试验,该菌发酵上清液仍有明显的抑菌活性;通过蛋白酶K和胰蛋白酶试验,证明该菌株产生的抑菌活性物质具有蛋白质性质,是一种细菌素。初步提纯该细菌素,其抑菌活性较适pH范围为2～4,120℃热处理30min后仍有70%的抑菌活性。经Trcine-SDS-PAGE试验分析该细菌素分子质量为4kDa。抑菌谱测定结果表明,该细菌素不仅对革兰氏阳性菌(单增李斯特氏菌、枯草芽孢杆菌、金黄色葡萄球菌等)有抑制作用,而且还抑制革兰氏阴性菌(大肠杆菌、沙门氏菌、恶臭假单胞菌、福氏志贺氏菌等),具有广谱的抑菌作用。通过菌落形态、生理生化特性试验和16S rRNA基因序列分析,鉴定该乳酸菌为植物乳杆菌,命名为Lactobacillus plantarum ZJ317。
Lactic acid bacteria isolated in the laboratory was identified.After eliminating some interference factors such as the organic acids,hydrogen peioxides and so on,the bacteriocin obtained from culture supernatant still remained active,and significant reduction in antimicrobial activity was observed after treatment with proteinase K and trypsin,confirming its proteinaceous nature.The bacteriocin showed high temperature stability up to 120 ℃ for 30 min with less loss in its activity,and had pH stability in the range of 2～4.The molecular weight of the bacteriocin is approximately 4.0 kDa by tricine-SDS-PAGE assay.And the bacteriocin also evidenced a broad spectrum of antibacterial activity against both Gram-negative and Gram-positive bacteria including Listeria monocytogenes,Bacillus subtilis,Staphylococcus aureus,Escherichia coli,Salmonella enterica,Pseudomonas aeruginosa,Shigella flexneri.Lactobacillus plantarum on the base of physiological and biochemical characteristics and 16S rRNA gene sequences,named as Lactobacillus plantarum ZJ317.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第3期181-186,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省重大科技专项(2007C12037)
浙江省自然科学基金杰出青年团队项目(R306425)
关键词
乳酸菌
鉴定
细菌素
抑菌特性
lactic acid bacteria
identification
bacteriocin
antimicrobial characteristic
作者简介
王小娜，女，1982年出生，硕士生
通讯作者：顾青
