摘要
目的:体外分离培养小型猪牙周膜细胞,为建立小型猪体内实验动物模型及进行牙周组织再生的研究奠定实验基础。方法:无菌拔除小型猪上下颌4颗切牙及4颗尖牙,采用组织块法原代培养小型猪牙周膜细胞并传代,绘制生长曲线,通过形态学和免疫组织化学染色方法对其进行鉴定。结果:组织块法成功培养小型猪牙周膜细胞,培养成功率为50%,其中切牙培养的成功率高达100%,尖牙培养成功率为0。细胞生长良好,呈梭形或多角形,免疫组织化学鉴定细胞抗波形丝蛋白阳性,抗角蛋白阴性,为中胚层来源的细胞。结论:小型猪的牙周膜细胞可成功培养,切牙的培养成功率较高,有望为牙周组织再生研究提供有用的细胞来源,小型猪牙周膜细胞可作为体内实验动物模型。
Objective To isolate and culture miniature pig periodontal ligament cells in vitro,pave the way for developing a animal model of miniature pig and provide experimental basis for periodontal regeneration.Methods 4 incisors and 4 canines were extracted in the condition of asepsis.The primary cells were isolated from miniature pig periodontal ligament and cultivated by tissue explant culture technique,then passaged when the cells completely covered the bottom of culture flasks.The growth curve was drawn.Morphological analysis and immunocytochemical analysis were used to characterize the cell lineage.Results The miniature pig periodontal ligament cells of primary culture were obtained with tissue cultivation,the success rate of primary culture was 50% and the highest successful rate（100%） was observed in incisor.The actively proliferating cells showed long spindle appearance.By immunocytochemical analysis,these cells were positive to antibodies against vimentin,and negative to antibodies against ceratin,indicating themselves to be mesoderm-derived fibroblasts.Conclusion The miniature pig periodontal ligament cells,which can be cultived successfully in vitro,especially the cells from incisor,may be the potential cells for periodontal regeneration in animal model.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2011年第3期475-478,I0004,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划重点项目资助课题(20090446)
关键词
小型猪
牙周膜细胞
细胞培养
组织块法
miniature pig
periodontal ligament cells
cell culture
tissue method
作者简介
赵斌（1983-），女，吉林省集安市人，医学硕士，主要从事口腔牙周病学基础与l临床研究。
[通信作者]林崇韬（Tel：043188796039，Email：linchongtao@sohu．com）
