摘要
目的探讨微波照射预处理对兔肝缺血再灌注(I/R)损伤的影响及机制。方法选择新西兰大白兔32只随机分为假手术组、微波照射组、微波照射后再灌注组、单纯再灌注组4组,每组各8例。均于术后2、4 h抽血查丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH),后处死并获取肝脏标本,检测各组肝组织匀浆脂质过氧化物丙二醛(ma-londi-aldehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)水平,并通过HE染色观察组织学变化。结果术后2、4 h微波照射组、微波照射后再灌注组、单纯再灌注组ALT、AST、LDH显著高于假手术组,其中单纯再灌注组高于微波照射后再灌注组,差异均有统计学意义(P<0.01);微波照射后再灌注组MDA浓度低于单纯再灌注组,SOD浓度高于单纯再灌注组,差异均有统计学意义(P<0.01);微波照射后再灌注组、单纯再灌注组NO、NOS浓度显著低于假手术组、微波照射组,其中单纯再灌注组又显著低于微波照射后再灌注组,差异亦均有统计学意义(P<0.01)。微波照射后再灌注组肝组织病理改变明显轻于单纯再灌注组。结论微波照射预处理通过减少MDA生成,提高SOD活性及NO、NOS含量,改善肝脏I/R损伤。
Objective To investigate the effect and mechanisms of microwave-irradiated preconditioning on the ischemia-reperfusion（I/R） liver injury in rabbits.Methods 32 rabbits were randomly divided into four groups： sham operation group,microwave-irradiated group,microwave-irradiated reperfusion group and reperfusion group（8 in each）.Then blood was obtained for assay of ALT,AST and LDH in 2 h and 4 h post-injury respectively.Livers were obtained from sacrified rabbits.NO,NOS,MDA,and SOD were measured in liver homogenate.Liver tissues were stained by HE.Results Serum levels of ALT,AST,LDH in microwave-irradiated group,microwave-irradiated reperfusion group and reperfusion group were higher than those in sham operation group,and the above mentioned indicators in reperfusion group were higher than those in microwave-irradiated reperfusion group（P0.01）.Compared with that in reperfusion group,MDA concentration in microwave-irradiated reperfusion group was lower,but SOD concentration was higher（P0.01）;The levels of NO,NOS in microwave-irradiated reperfusion group and reperfusion group were lower than those in sham operation group and microwave-irradiated group（P0.01）,and the above mentioned indicators in reperfusion group were higher than those in microwave-irradiated reperfusion group（P0.01）.Morphological change in microwave-irradiated reperfusion group was slighter than those in reperfusion group.Conclusion Microwave-irradiated preconditioning can improve I/R injury in rabbit liver by reducing MDA production,elevating the activity of SOD,and increasing the contents of NO and NOS.
出处
《临床误诊误治》
2011年第6期9-11,F0003,共4页
Clinical Misdiagnosis & Mistherapy
关键词
微波
再灌注损伤
一氧化氮
一氧化氮合酶
丙二醛
超氧化物歧化酶
Microwav
Reperfusion injury
Nitric oxide
Nitric-Oxide Synthase
Malondialdehyde
Superoxide dismutase
