摘要
目的探讨新疆地区紫色毛癣菌的基因特点，为新疆儿童头癣致病菌的分子流行病学研究提供依据。方法用5种限制性内切酶HaeⅢ，BglⅠ，MspI，DdeI及MboI，采用PCR限制性片段长度多态性（RFLP）法对分离于新疆儿童头癣的30株紫色毛癣菌的rDNA非转录间隔（Nrrs）区进行基因分型，并与北京的9株、台湾的2株紫色毛癣菌做比较。结果内切酶DdeI将全部41株菌分为12个基因型。新疆的30株菌分为10个基因型，其中17株菌分为D、F、G、H、I、J、K7个基因型，与北京和台湾菌株间有明显基因差异，其余13株菌分3个基因型，与北京和台湾菌株有基因同源性。结论新疆地区儿童头癣分离的紫色毛癣菌既有独特基因型，又与北京、台湾菌株有基因同源性，体现了新疆紫色毛癣菌的基因多态性。
Objective To analyze the genotypic characteristics of T. violaceum, and offer evidence for molecular epidemiological study of causative agents of pediatric tinea capitis, in Xinjiang Uygur Autonomous Region. Methods The PCR-restriction fragment length polymorphisim （RFLP） of the nontranscribed spacer region of ribosomal DNA （rDNA-NTS） was assessed by using 5 restriction enzymes, including Hae Ⅲ, Bgl I, MspI, DdeI and MboI, for 30 clinical isolates of T. violaceum from children with tinea capitis in Xinjiang Uygur Autonomous Region. Nine strains of T. violaeeum from Beijing and 2 strains from Taiwan served as the control. Results All the 41 strains of T. violaeeum were classified into 12 genotypes with the restriction enzyme DdeI. Ten genotypes were revealed in the 30 strains from Xinjiang; among them, 17 strains showed 7 different geno- types （D, F, G, H, I, J and K） with a high intraspecies diversity in comparison with the Beijing and Taiwan isolates; the remaining 13 strains from Xinjiang showed 3 genotypes, which were shared by the Beijing and Taiwan isolates. Conclusions The T. violaceum isolates from children with tinea capitis in Xinjiang Uygur Autonomous Region have unique genotypes, but also show genetic homogeneity with the strains from Beijing and Taiwan. hinting the genetic diversitv of T. violaceum.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2011年第6期387-389,共3页
Chinese Journal of Dermatology
基金
基金项目：国家自然科学基金（30960351）,新疆少数民族科技人才特殊培养计划科研项目（200823113）
关键词
癣
头
紫色毛癣菌
DNA指纹法
Tinea capitis
Trichophyton violaceum
DNA fingerprinting
作者简介
通信作者：帕丽达·阿布利孜，Email：palidaa@yahoo．com．cn
