摘要
目的:观察SD大鼠视神经挫伤后樟柳碱(anisodine,AT3)联合地塞米松(dexamethasone,Dex)治疗对闪光视觉诱发电位(flash visual evoked potential,F-VEP)和视网膜电图(electroretinogram,ERG)的影响。方法:成年雌性SD大鼠30只,均以左眼为实验眼,采用随机数字法分为A,B,C共3组,利用压力恒定的无创反向镊夹持大鼠视神经6s建立视神经挫伤模型,A组建立模型前1d开始每日给予尾静脉注射0.25mL(0.5mg)Dex,同时左眼球后注射0.25mLAT-3;B组尾静脉注射同A组,但球后注射生理盐水;C组静脉及球后均给予生理盐水。记录并比较3组大鼠建模前及建模后0.5,1,7,14d的F-VEP的P1波和ERG的b波振幅和峰潜时变化。结果:C组视神经损伤0.5d后,F-VEP振幅值降低与正常相比有显著性差异,峰潜时在1d后与正常相比有统计学意义,并且在观察时间内随时间其峰潜时和波幅呈进行性下降。B组在7d后F-VEP振幅值明显高于与对照组(P=0.031),峰潜时短于对照组(P=0.012),C组在7d后F-VEP振幅值明显高于对照组(P=0.042),峰潜时短于对照组(P=0.019)。结论:AT3联合Dex对大鼠视神经挫伤后神经传导功能的恢复较单纯应用Dex明显。
AIM：To observe the impact of anisodine （AT3） combined with dexamethasone （Dex） treatment on the flash visual evoked potentials （ F-VEP） and electroretinogram （ERG） of SD rats after optic nerve contusionMETHODS： Thirty adult female SD rats,taken their left eyes as experimental eyes, were divided into A, B, C groups using random numbers, and gripped optic nerve 6 seconds with noninvasive pressure-constant reverse tweezers to establish optic nerve contusion models.Group A was given caudal vein injection of 0.25mL （0.5mg） Dex one day before setting up the model, as well as retrobulbar injection of 0.25mL AT3 in the left eye; Caudal veins of group B was injected the same amount of normal saline by retrobulbar injection; Group C was given both intravenous and retrobulbar injection of normal saline. P1 wave of the F-VEP and b-wave amplitude and peak latency changes of ERG in 3 groups were recorded and compared before and 0.5,1 day,7,14 days after modeling.RESULTS： In group C , 0.5 day after optic nerve injury, F-VEP amplitude decreased significantly when compared with the normal . Peak latency 1 day after modeling had statistical significance when compared with the normal, and peak latency and amplitude decreased progressively during the observation time. In group B 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group （P=0.031）, peak latency shorter than the control group （P=0.012）. In group C 7 days after modeling, the F-VEP amplitude was significantly higher than that in the control group （P=0.042）, peak latency shorter than the control group （P=0.019）.CONCLUSION：Curative effect of AT3 combined with Dex on the recovery of nerve conduction after optic nerve contusion in rats is significantly higher than that of alone applications of Dex.
出处
《国际眼科杂志》
CAS
2011年第6期961-963,共3页
International Eye Science
作者简介
作者简介：丁同鹏，硕士，主治医师，研究方向：视网膜与视神经疾病通讯作者：丁同鹏．dingyaayaa@yahoo．cn
