摘要
目的为生物制品生产及研究用Vero细胞体系传代过程中的遗传安全性评价提供实验数据。方法对Vero细胞进行传代与冻存，并对不同代次Vero细胞进行体外与体内致瘤性检测，同时对不同代次Ver6细胞的蛋白质表达进行差异性分析。结果Vero细胞传代至270代并建立了14个细胞冻存库；不同代次Vero细胞体外与体内致瘤性检测结果均为阴性；200代以上的Vero细胞蛋白质表达水平有所变化。结论Vero细胞传代过程中虽然体外与体内致瘤性检测结果为阴性，但蛋白质组学分析有差异性蛋白表达，这为Vero细胞传代过程中的遗传安全性评价提供了实验参考。
Objective To produce an experimental information for the safety assessment of Vero cells during subculture. Methods Passage and freeze on Vero cells, and Vero cells in different passages in vitro and in vivo tumorigenicity were tested. The protein expression of different Vero cell passages was analyzed. Results Veto cells passaged to p270 and 14 cell banks were developed and stored for future evaluation. In vitro and in vivo tumorigenicity test results of Vero cells in different passages were negative. Conclusion Although the tumorigenicity test results in vitro and in vivo process were negative, the protein expression of more than p200 Vero cells were changed, which produced the experimental reference for the safety evaluation of the process during the Vero cell passage.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2011年第5期456-461,共6页
Chinese Journal of Microbiology and Immunology
基金
国家科技支撑计划（2008BA154804-02）
作者简介
通信作者：王辉，Email：wh6247@sohu．com，电话：010-65762911
