摘要
使用富集、稀释涂平板的方法从制药、染料、化工及精细化工、食品等多家工厂的混合高浓度难降解化工废水中筛选到8株细菌,经16S rDNA鉴定为Bacillus aquimaris、Enterobacter、Pseudomonas putida、Microbacterium、Agrobacterium、Alpha proteobacterium、Planococcus rifietoensis,其中两株细菌为Bacillus aquimaris。对各单一菌种生长条件的研究表明,各菌种生长的最适pH为7,最适接种量为15%(v/v),最适生长温度为37℃,其中Pseudomonas putida的最适生长温度为30℃。在最适生长条件下,研究了各单一菌种及不同组合的混合菌种对废水的降解效果,结果表明在单一菌种中Pseudomonas putida对废水的降解效果最优,混合菌的降解效果优于单一菌种的降解效果,混合菌可将废水COD由1 249 mg/L降至97 mg/L,比普通活性污泥对废水的去除率提高了14.7%。
Eight bacteria were screened with the method of enrichment and dilution from the mixed wastewater of pharmaceutical,dye,chemical and fine chemical,food industries with high concentration and hard-degradation.The eight bacteria were identified as Bacillus aquimaris,Enterobacter,Pseudomonas putida,Microbacterium,Agrobacterium,Alpha proteobacterium,Planococcus rifietoensis by 16S rDNA identification,two bacteria of them were Bacillus aquimaris.Study on the growth condition of the eight bacteria showed that the optimal pH was 7,the optimal inoculation amount was 15%（v/v） and the optimal growth temperature was 37℃,but Pseudomonas putida was 30℃.The study on degradation of the wastewater by the each single bacterium and the mixed bacteria was carried under the optimal growth condition.The results showed that degradation efficiency of Pseudomonas putida was better than the other single bacterium,and degradation efficiency of the mixed bacteria was better than any single bacterium.COD of the wastewater was degraded from 1 249 mg/L to 97 mg/L by the mixed bacteria,and removal rate was increased to 14.7%,better than the conventional activated sludge.
出处
《城市环境与城市生态》
CAS
CSCD
2011年第3期30-33,共4页
Urban Environment & Urban Ecology
基金
国家"十一五"重大科技支撑计划课题(2008ZX07314-001)
关键词
菌种筛选
16S
rDNA鉴定
降解条件
单一菌种
混合菌
bacterial screening
16S rDNA identification
degradation condition
single bacterium
mixed bacteria
作者简介
吕晶华（1983-）女，河北邯郸人，硕士，助理工程师，主要研究方向为水处理技术一环境微生物，发表论文1篇。
