摘要
目的:观察肝乐颗粒(Ganle Keli,GL)对肝纤维化大鼠的保护作用。方法:除正常组外,其余各组采用四氯化碳诱导肝纤维化模型,每周2次,连续12周。于造模第7周起,给药组分别ig给予相应的受试药物,正常组和模型组给予等量溶媒,疗程6周。实验结束后,股动脉采血,比色法测定血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)活性、羟脯氨酸(Hyp)含量;同时取固定部位肝脏组织,HE染色;免疫组化技术测定肝组织中核因子-κB(NF-κB)蛋白的表达,RT-PCR技术测定肿瘤坏死因子-α(TNF-α)mRNA的表达。结果:肝乐颗粒1.5,3,6 g·kg-1各剂量组能明显降低肝纤维化大鼠ALT,AST,MDA,Hyp含量,升高SOD水平;减轻大鼠肝纤维化增生程度的同时抑制肝组织中NF-κB蛋白和TNF-αmRNA的表达。结论:肝乐颗粒对CCl4诱导的肝纤维化大鼠有很好的保护作用。
Objective：To study the protection of Ganle Keli（GL） on liver fibrosis in rats.Method：Rat liver fibrosis was induced by CCl4 twice a week for 12 weeks.GL was used daily via lavage starting from the 7th week of CCl4application.Contents of ALT,AST,MDA,SOD and Hyp were determinate by chromatometry.Liver samples were collected after experiments,stained by HE and immuninochemistry of NF-κB.Moreover TNF-αmRNA expression in liver tissue was detected by RT-PCR technology.Result：GL could significantly reduce ALT,AST and MDA contents,increase the level of SOD.Meanwhile GL could effectively prevent the expression of NF-κB and the degree of hepatic fibrosis,and significantly reduce TNF-αmRNA in rats with liver fibrosis.Conclusion：GL has protective effect on liver fibrosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第12期167-170,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
安徽中医学院临床科研基金(2010LC-025A)
作者简介
[第一作者]姜辉，硕士，药师，从事中药药理研究，Tel：0551—2838553，E—mail：jhanbing@163．com.
