摘要
利用33对多态性SSR引物对84个茶树品种进行亲缘关系和遗传多样性分析,并基于SSR数据,对供试品种进行UPGMA遗传相似性聚类分析。结果表明:参试品种具有较高的遗传多样性(共检测到94个等位位点,每个引物2~4个,平均2.85个;引物多态性信息含量为0.20~0.79,平均值为0.56;可观测等位位点数最多9个,最少3个,平均4.79个;杂合度观测值为0.08~0.97,平均值为0.53;杂合度期望值为0.14~0.86,平均值为0.62;群体内的Shannon指数平均值为1.17);聚类分析结果显示,阿萨姆茶等6个品种各自形成了单独的分支,其余78个品种聚成1个大类群,在相似系数0.54处,这个大类群又可分为6个亚类群。
The 33 pairs of simple sequence repeats (SSR) primers were used to analyze the genetic polymorphism and genetic relationship of 84 tea cultivars. Totally 94 alleles were amplified using 33 SSR primers, the number of alleles per primer ranged from 2 to 4, averaged 2.85. The polymorphism information content (PIC) varied from 0.20 to 0.79, averaged 0.56. The observed heterozygosity (Ho) ranged from 0.08 to 0.97 and the expected heterozygosity varied from 0.14 to 0.86, averaged 0.53 and 0.62, respectively. The Shannon index of all accessions is 1.17. All of the results showed that the genetic polymorphism of all accessions were relatively abundant. The 84 accessions were classified into 6 groups based on the UPGMA method with the similarity coefficient at 0.54.
出处
《湖南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第3期260-266,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
科技部农业科技成果转化基金项目(2010GB2D200320)
关键词
茶树
遗传多样性
亲缘关系
简单重复序列
非加权组平均法
tea plant
genetic diversity
genetic relationship
simple sequence repeats (SSR)
unweighted pair-groupmethod with arithmetic averages (UPGMA)
作者简介
王旭(1972-),男,湖南邵阳人,副研究员,主要从事茶树资源及育种研究,wang.xul972@163.com