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H3N2 SIV河南株非结构蛋白1(NS1)重组的纯化及Dot-ELISA的建立 被引量：1

Purification of the Recombinant NS1 Protein of H3N2 SIV in He′nan Province and Preliminary Establishment of a Dot-ELISA
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摘要 为建立鉴别猪流感病毒感染猪和灭活疫苗免疫猪的诊断方法,利用pET-28a载体在大肠杆菌BL21中表达H3N2 SIV河南株NS1,并通过Ni2+-NTA亲和色谱法纯化His-NS1融合蛋白.用SDS-PAGE,Western blot-ting和Dot-ELISA分析纯化蛋白,表明重组NS1能与猪流感病毒感染猪血清进行特异性反应,而不与灭活疫苗免疫猪血清反应.结果表明重组蛋白能作为区别猪流感病毒感染猪和灭活疫苗免疫猪的诊断抗原,为控制猪群中的流感病毒奠定了基础. To develope a diagnostic method of differentiating the infected from the vaccinated animals,NS1 of Swine influenza virus（SIV）isolated in Henan was expressed in BL21（pET-28a）,and His-NS1 fusion protein was purified by Ni2＋-NTA affinity chromatography.The purified NS1 protein was analyzed by SDS-PAGE,Western blotting and dot-ELISA.Results showed that the recombinant NS1 protein can react specifically with sera from pig infected with SIV,but not sera from pig immuned with inactived vaccine.It is concluded that the recombinant NS1 protein can be used as a diagnostic antigen to differentiate the infected from the vaccinated pigs,providing the basis for control of influenza viruses in pigs.
作者 赵朴 郑玉姝 贾贝贝 赵坤 姚四新 王三虎 刘兴友 刘丽艳
机构地区 河南科技学院动物科学学院 动物疫病和残留物防控河南省高校工程技术研究中心
出处 《西南师范大学学报（自然科学版）》 CAS CSCD 北大核心 2011年第3期190-193,共4页 Journal of Southwest China Normal University(Natural Science Edition)
基金 河南科技学院青年基金资助项目(7044)
关键词 猪流感病毒 非结构蛋白1 纯化 鉴别诊断 swine influenza virus nonstructura1 protein 1 purification differential diagnosis
分类号 S852.651 [农业科学—基础兽医学]
作者简介 赵朴（1975-），男，河南南阳人，讲师，硕士，主要从事动物病毒学与免疫学研究．
  • 相关文献

参考文献10

  • 1GUTIERREZ-MARTIN C B, RODRIGUEZ-DELGADO O, ALVAREZ-NISTAL D, et al. Simultaneous Serological Ev- idence of Actinobaeillus Pleuropneumoniae, PRRS, Aujeszky's Disease and Influenza Viruses in Spanish Finishing Pigs [J]. Res Vet Sci, 2000, 68(1): 9-13.
  • 2ITO T, COUCEIRO J, KELM S, et al. Molecular Basis for the Generation in Pigs of Influenza A Virus with Pandemic Potential[J]. J Virol, 1998, 72(9): 7367-7373.
  • 3BROWN I H, HARRIS P A, MCCAULEY J W, et al. Multiple Genetic Reassortment of Avian and Human Influenza A Viruses in European Pigs, Resulting in the Emergence of a H1N2 Virus of Novel Genotype [J]. J Gen Virol, 1998, 79(12):2947-2955.
  • 4RICHT J A, LAGER K M, JANKE B H, et al. Pathogenic and Antigenic Properties of Phylogenetically Distinct Reas sortant H3N2 Swine Influenza Viruses Cocirculating in the United States[J]. J Clin Microbiol, 2003, 41 (7): 3198-3205.
  • 5MA Wen-jun, GRAMER M, ROSSOW K, et al. Isolation and Genetic Characterization of New Reassortant H3N1 Swine Influenza Virus From Pigs in the Midwestern United States [J]. J Virol, 2006, 80(10) : 5092-5096.
  • 6NAFFAKH N, VAN DER WERF S. April 2009: An Outbreak of Swine-origin Influenza A(H1N1) Virus with Evidence for Human-to-human Transmission [J]. Microbes Infect, 2009, 11(8- 9) : 725- 728.
  • 7VINCENT A L, MA Wen-jun, LAGER K M, et al. Efficacy of Intranasal Administration of a Truncated NS1 Modified Live Influenza Virus Vaccine in Swine [J]. Vaccine, 2007, 25(47): 7999-8009.
  • 8RICHT J A, LEKCHAROENSUK P, LAGER K M, et al. Vaccination of Pigs Against Swine Influenza Viruses By U- sing a NSl-truncated Modified Live-virus Vaccine [J]. J Virol, 2006 , 80(22): 11009-11018.
  • 9赵朴,郑玉姝,李海燕.猪流感--不容忽视的潜在人兽共患病[J].中国兽医杂志,2008,44(9):54-56. 被引量：13
  • 10TUMPEY T M, ALVAREZ R, SWAYNE D E. Diagnostic Approach for Differentiating Infected from Vaccinated Poul- try on the Basis of Antibodies to NS1, the Nonstructural Protein of Influenza A Virus [J]. J Clin Microbiol, 2005, 43(2): 676-683.

二级参考文献21

  • 1Choi Y K, Goyal S M, Joo H S. Prevalence of swine influenza virus subtypes on swine farms in the United States [J]. Arch Virol, 2002,147(6) : 1209-1220.
  • 2Maldonado J, van Reeth K, Riera P, et al. Evidence of the concurrent circulation of HIN2, H1N1 and H3N2 influenza A viruses in densely populated pig areas in Spain[J]. Vet J, 2006,172(2) :377-381.
  • 3Nowotny N, Mostl K, Maderbacher R, et al. Serological studies in Austrian fattening pigs with respiratory disorders [J]. ActaVet Hung, 1994, 42(2-3):377 -379.
  • 4Groschup M H, Brun A, Haas B. Serological studies on the potential synergism of porcine reproductive and respiratory syndrome virus and influenza-, corona- and paramyxoviruses in the induction of respiratory symptoms in swine[J]. Zentralbl Veterinarmed B, 1993 ,40(9 10):681- 689.
  • 5Loeffen W L, Kamp E M, Stockhofe-Zurwieden N,etal. Survey of infectious agents involved in acute respiratory disease in finishing pigs[J]. Vet Rec, 1999 , 145(5):123-129.
  • 6Jung K, Chae C. First outbreak of respiratory disease associated with swine influenza H1N2 virus in pigs in Korea[J]. J Vet Diagn Invest, 2005, 17(2) :176 -178.
  • 7Jung T, Choi C, Chae C. Localization of swine influenza virus in naturally infected pigs[J]. Vet Pathol, 2002 ,9(1) :10-16.
  • 8Kay R M, Done S H, Paton D J. Effect of sequential porcine reproductive and respiratory syndrome and swine influenza on the growth and performance of finishing pigs[J]. Vet Rec, 1994, 135(9):199-204.
  • 9Elbers A R, Tielen M J, Cromwijk W A,etal. Sero epidemio logical screening of pig sera collected at the slaughterhouse to detect herds infected with Aujeszky's disease virus, porcine influenza virus and Actinobacillus (Haernophilus) pleuropneumoniae in the framework of an integrated quality control (IQC) system[J]. Vet Q, 1990, 12(4):221-230.
  • 10Wesley R D. Exposure of sero-positive gilts to swine influenza virus may cause a few stillbirths per litter[J]. Can J Vet Res, 2004, 68(3) :215-217.

共引文献12

同被引文献9

  • 1Smith GJ,Vijaykrishna D,Bahl J ,et al. Origins and evolutionary ge- nomics of the swine-origin H1 N1 influenza A epidemic [J ], Nature, 2009,459 ( 7250 ) : 1122 - 1125.
  • 2Heiny AT, Miotto O, Srinivasan KN, et al. Evolutionarily con- served protein sequences of influenza a viruses, avian and hu- man, as vaccine targets [ J ]. PLoS ONE, 2007,2 ( 11 ) : el 190.
  • 3Ito T, Couceiro J, Kelm S, et al. Molecular basis for the genera- tion in pigs of influenza A virus with pandemic potential [ J ]. J Virol, 1998,72:7367 - 7373.
  • 4Brown IH, Harris PA, McCauley JW, et al. Multiple genetic reas- sortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H 1 N2 virus of novel geno- type [ J ]. J Gen Virol, 1998,79 ( Pt 12 ) :2947 - 2955.
  • 5Zhu X, Yu W, McBride R, et al. Hemagglutinin homologue from H17N10 bat influenza virus exhibits divergent receptor-binding and pH-dependent fusion activities [ J ]. Proc Natl Acad Sci USA,2013,110(4) : 1458 - 1463.
  • 6Catragher DM,Kaminski DA,Moquin A,et al. A novel role for non- neutralizing antibodies against nucleoprotein in facilitating resistance to influenza virus[J]. J Immunol,2008,181 (6) :4168 -4176.
  • 7赵朴,郑玉姝,李海燕.猪流感--不容忽视的潜在人兽共患病[J].中国兽医杂志,2008,44(9):54-56. 被引量：13
  • 8赵朴,郑玉姝,贾贝贝,乔传玲,陈化兰,刘兴友,李海燕.猪流感病毒HA基因的克隆及杆状病毒转移载体的构建[J].生物技术通报,2009,25(9):97-100. 被引量：4
  • 9龚震宇,陈恩富,吕华坤,凌锋,余昭,柴程良,孙继民,陈直平.人感染甲型H7N9禽流感疫情及动物源性疾病防控分析[J].中国公共卫生,2014,30(1):35-37. 被引量：8

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西南师范大学学报（自然科学版）
2011年 第3期

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