摘要
【目的】为利用新疆一枝蒿研制新型无公害植物杀虫药剂奠定基础。【方法】采用97%乙醇对新疆一枝蒿(Artemisia rupestris)和黄花蒿(Artemisia annua)进行了总提,将所得粗提物对棉花重要害虫—棉铃虫(Helicoverpa armigera)与棉蚜(Aphis gossypii)进行了生物活性测定,同时还使用新疆一枝蒿粗提物对棉铃虫开展了室内、室外的驱避实验。【结果】(1)新疆一枝蒿与黄花蒿粗提物对棉铃虫半致死浓度LC50分别为3.515 7与4.781 1 mL/100g;(2)新疆一枝蒿与黄花蒿粗提物对棉蚜半致死浓度LC50分别为0.956与2.047 4 g/100 mL;(3)新疆一枝蒿粗提物对棉铃虫室内室外趋避率分别为28.39%与80.68%。【结论】新疆一枝蒿粗提物对棉铃虫与棉蚜的毒杀作用较好并高于黄花蒿粗提物,且新疆一枝蒿粗提物对棉铃虫有驱避和生长抑制等作用。
[ Objective] This research can be a foundation for the development of new pollution-free botanical insecticides. [ Method] Two types of coarse solutions from Artemisia rupestris and Artemisia annua were extracted with 97 % ethanol, and toxicities of the two extracts to cotton bollworm and cotton aphid were determined, and expellent effect of Artemisia rupestris to laying eggs of cotton bollworms in both the laboratory and the field were also studied. [Result] The results showed：（ 1 ） The LC50 of extracts from Artemisia rupestris and Artemisia annua to cotton bollworm are 0.956 and 2.047 4 g/100mL; （2） The LC50 of extracts from Artemisia rupestris and Artemisia annua to cotton aphid are 0.956 and 2.047 4 g/100mL; （3） The repellency of extracts of Artemisia rupestris on laying eggs of cotton bollworm in the laboratory and in the field are 28.39% and 80.68%. [ Conclusion] The findings demonstrate that the extracting coarse solutions from Artemisia rupestris are better than that from Artemisia annua as to the killing effect on cotton bollworm and cotton aphid, and it had an expellent effect on laying eggs of cotton bollworms in both the laboratory and the field.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2011年第5期889-895,共7页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区科技支疆项目(200991119)
新疆生物资源基因工程重点实验室项目(XJDX0201-2007-04)
关键词
新疆一枝蒿
黄花蒿
棉铃虫
棉蚜
粗提物
杀虫生物活性
Artemisia rupestris
Artemisia annua
Helicoverpa armigera
Aphis gossypii
extracting coarse solution
poisonous activity
作者简介
何海（1987-），男，新疆乌鲁木齐人，硕士研究生，研究方向为昆虫毒理，（E—mail）hehai0528@sohu．com
通讯作者：刘小宁（1971-），女，陕西人，副教授，博士，硕士生导师，研究方向为农业昆虫与害虫防治，（E-mail）liuxn0103@sina．com
