摘要
目的:观察升降散对大鼠系膜增生性肾小球肾炎的治疗作用。方法:40只大鼠随机分为对照组、模型组、洛汀新组、升降散组。除对照组外,其余各组动物均制备慢性系膜增生性肾小球肾炎模型,药物干预两组于造模第5周起ig升降散5.4 g·kg-1·d-1,洛汀新1.17 mg·kg-1·d-1,共8周。于实验前、4周末、12周末测定24 h尿蛋白量;12周末分别处死各组大鼠,观察肾脏病理变化,免疫组织化学法检测肾脏组织中NF-κB表达。结果:模型组24 h尿蛋白较对照组升高(P<0.01);药物干预两组较模型组减少(P<0.05)。模型组大鼠肾小球及肾间质NF-κB表达明显增加,且多在细胞核表达,升降散组及洛汀新组NF-κB表达较模型组减少。结论:升降散能减少肾炎大鼠尿蛋白排泄量,降低肾组织NF-κB表达,抑制肾小球系膜细胞与基质增生。
Objective：To observe the therapeutic effects of Shengjiangsan on mesangial proliferative glomerulonephritis in rats.Method：Forty normal rats were randomly divided into control group,model group,Shengjiangsan group and Lotensin group.Except of the control group,the rats in last three groups were made as model of mesangial proliferative glomerulonephritis.From the fifth week,rats in Shengjiangsan group and Lotensin group were feed with Shengjiangsan 5.4 g·kg-1·d-1,and Lotensin 1.17 mg·kg-1·d-1 for eight weeks.respectively.Twenty four hours urine protein excretion was tested respectively before experiment,the forth week and the 12th week.After the 12 week,all the rats were executed.Kidneys were conserved to observe their pathological changes in different groups.Meanwhile NF-κB expression in kidney tissue was detected by immunohistochemistry.Result：The level of 24 hours urine protein（UP）,in the model group was higher compared with contral group（P0.01）,The levels in Shengjiangsan and Lotensin group were decreased（P0.05）.NF-κB expression in both glomerulus and renal interstitium in rats of model group was significant,and heavily expressed in nucleolus.But expressions in Shengjiangsan and Lotensin group were weaker.Conclusion：Shengjiangsan can reduce the urine protein excretion in rats with mesangial proliferative glomerulonephritis,decrease the expression of NF-κB in kidney tissue,and relieve hyperplasy of intercapillary cells and ground substance.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第10期190-193,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
青岛市中医管理局资助项目[2009-zyy007]
作者简介
[通讯作者]于俊生，主任NN／教授，医学博士，从事肾脏病研究，Tel：0532—83777005，E-mail：yujs007@163．com
