摘要
目的:通过对没食子酸(Gallicacid,GA)在体外清除自由基能力的评价,为其抗氧化应用提供实验依据。方法:采用分光光度比色法,分别测定药物对2,2-联苯-1-苦基肼(2,2-Diphenyl-1-picrylhydrazyl,DPPH)自由基、过氧化氢和羟自由基的清除作用,以及对脂质过氧化的抑制作用,采用Griess法测定药物对一氧化氮的清除作用。并与公认的自由基清除剂维生素C(Vitamin C,Vc)同时实验进行比较。结果:GA在所选浓度范围内(5~50μg/ml)对DPPH自由基就有明显的清除作用,清除率为(20.65±2.67)%^(87.98±4.12)%,明显高于Vc的清除作用,并呈一定的浓度依赖关系;对一氧化氮的清除率在所选浓度范围内50~400μg/ml达到(10.45±3.45)%^(40.43±2.76)%,其清除能力很强,只是略低于Vc;对羟自由基的清除率在所选浓度范围内(50~400μg/ml)为(47.32±3.26)%^(67.25±2.99)%,其清除作用明显并且高于Vc。而对过氧化氢清除作用并不明显。不同浓度的GA 50~400μg/ml对脂质过氧化有抑制作用,抑制率为(48.65±5.21)%^(80.03±5.64)%,其抑制效应远远高于Vc。结论:GA在体外对不同自由基具有较强的清除能力,这可能是其在体内起到抗氧化作用的重要因素之一。
Objective：To introduce the free radical scavenging and anti-lipoperoxidative activities of gallic acid in vitro and to obtain the experimental data of antioxidant activities.Methods：Using spectrophotometry,the effect of gallic acid in scavenging free radical ·OH,free radical ·2,2 Diphenyl 1 picrylhydrazyl and H2O2 were determined.The antilipoperoxidative effect of gallic acid was also evaluated by spectrophotometry.The scanvenging level of NO was tested by Griess.The experiments were studied respectively and compared with vitamin c（Vc）.Results： Gallic acid in the suitable concentration 5-50 μg/ml could scavenge 2,2-Diphenyl-1-picrylhydrazyl by（20.65±2.67）%^（87.98±4.12）% in dose dependent manner.The scavenging capability of gallic acid for 2,1-Diphenyl-1-picrylhydrazyl was significantly higher than that of vitamin c.Gallic acid in the suitable concentration 50~400 μg/ml could scavenge NO by（10.45±3.45）%^（40.43±2.76）%,which was slightly lower than vitamin c.Gallic acid in the suitable concentration 50~400 μg/ml could scavenge hydroxylion by（47.32±3.26）%^（67.25±2.99）%,which was significantly higher than vitamin c.The capability of gallic acid for scavenging against H2O2 in vitro was weak.Gallic acid in various concentrations 50~400 μg/ml could inhibit lipid peroxidation effectively and the rate of inhibition was（48.65±5.21）%^（80.03±5.64）%,which was significantly higher than that of vitamin c.Conclusion：There is powerful scavenging effect of gallic acid against different free radicals in vitro.This might be one of the reasons for its antioxidant activities in vivo.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第3期319-322,共4页
Journal of Chongqing Medical University
基金
重庆市自然科学基金资助项目(编号:CSTC
2009BA5083)
重庆医科大学重点基金资助项目(编号:XBED200806)
关键词
没食子酸
抗氧化
自由基
清除率
gallic acid
anti-oxidant
free radical
scavenging rate
作者简介
谢晓艳（1985-），女，硕士，
于超，男，教授，Email；yuchaom@163．com。
