摘要
目的:研究电离辐射对体外胃腺癌细胞增殖、凋亡及周期的影响。方法:^(60)Coγ射线单次照射胃腺癌BGC-823细胞,照射剂量分别为0、2、4 Gy,利用MTT和克隆形成率实验检测各剂量组BGC-823的增殖能力;同时利用Annexin-V/PI双染法检测各剂量组BGC-823细胞的凋亡;用PI单染测定各剂量组BGC-823的细胞周期。结果:与0 Gy组相比,2、4Gy ^(60)Coγ射线照射可显著抑制胃腺癌BGC-823细胞的增殖(P<0.05),诱导BGC-823细胞在照射后依次出现S期和G_2/M期阻滞。4Gy ^(60)Coγ射线照射后48h,凋亡细胞比例显著高于对照组(P<0.05)。结论:^(60)Coγ射线照射可抑制胃腺癌细胞BGC-823增殖、诱导胃癌细胞出现周期阻滞和细胞凋亡。
OBJECTIVE：To study the effects of ionizing radiation on the proliferation,apoptosis and cell cycle of gastric adenocarcinoma cells in vitro.METHODS：After gastric adenocarcinoma BGC-823 cells were irradiated by 60Coγ-rays with a single dose of 0,2 or 4 Gy,the proliferation ability of BGC-823 cells in every dose group was evaluated by MTT and cloning efficiency assay.Apoptosis and cell cycle were further investigated by flow cytometry with Annexin-V/PI double staining and PI staining,respectively.RESULTS：Compared with the 0 Gy group,proliferation of gastric adenocarcinoma cells was inhibited significantly after doses of 2 and 4 Gyγ-rays irradiation（P〈0.05）. Furthermore,the cells were arrested in S then G_2/M phases afterγ-rays irradiation of 2 and 4 Gy.Finally,compared with 0 Gy group,the percentage of apoptotic cells in 4 Gy dose group increased significantly 48 h after irradiation（P〈0.05）. CONCLUSION：Radiation treatment of gastric adenocarcinoma BGC-823 cells with ^（60）Coγ-rays could inhibit cell proliferation,induce cell cycle arrest and promote apoptosis.
出处
《癌变．畸变．突变》
CAS
CSCD
2011年第2期119-122,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(30870749
30570551)
关键词
胃癌
辐射
增殖
凋亡
细胞周期
gastric adenocarcinoma
radiation
proliferation
apoptosis
cell cycle
作者简介
高玲（1978-），女，新疆人，助理研究员，研究方向：放射医学。E—mail：gaolinglinggao@yahoo．com．cn
Correspondence to： LIUQing-jie, E-mail： qjliu@nirp.cn
