摘要
从土壤中筛选到一株产β-葡萄糖苷酶的菌株FYS-9,其发酵可将京尼平苷转化为京尼平。通过菌落形态特征、孢子和菌丝显微观察及18S rDNA基因序列分析,初步鉴定该菌株为泡盛曲霉(Aspergillus awamori)。以FYS-9为出发菌株,通过紫外线、原生质体诱变,选育得到一株高产β-葡萄糖苷酶的突变株70C-3,该突变株发酵产酶平均酶活力达到36.15 IU/mL,比FYS-9提高39.4%。对突变株70C-3发酵转化京尼平苷条件的优化结果表明,在京尼平苷添加量1.5%,30℃、150r/min转化24h的条件下,京尼平苷的转化率达到97.7%,京尼平的产量可达8.5g/L。
A strain of fungus FYS-9 for producing genipin from β-glucosidase was screened from soil.This strain was identified as Aspergillus awamori according to its morphology characteristics and 18S rDNA gene sequence.Strain FYS-9 was utilized as the original strain to obtain another isolated mutant 70C-3.The β-glucosidase activity of strain 70C-3 was 36.15 IU/mL,an improvement of 39.4% compared with the original strain.The optimal conditions were found to be fermentation for 24h with addition of 1.5% geniposide at 30 ℃ with shaking at 150r/min.The conversion of geniposide reached 97.7% and the yield of genipin reached 8.5g/L.
出处
《北京化工大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期81-86,共6页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
作者简介
男，1986年生，硕士生
通讯联系人E-mail：chenchang@mail．buct．edu．cn
