摘要
【目的】观察精制蟒蛇油对D-半乳糖所致衰老小鼠皮肤组织的延缓衰老作用。【方法】选用昆明种小鼠,随机分为蟒蛇油高、中、低剂量组(中药高、中、低剂量组,剂量分别为10、5、2.5 mL/kg),维生素E组(剂量为50 mg/只),模型组和正常对照组;除正常对照组外,其他组均采用颈背部皮下注射D-半乳糖(剂量为1 g/kg)法复制亚急性衰老模型,同时各组皮肤涂布给药,连续42 d。取各组小鼠背部皮肤检测含水量、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,并进行组织病理学检查。【结果】模型组皮肤含水量及SOD活力降低,MDA含量升高,与正常对照组比较差异具有显著性意义(均P<0.01);中药高、中、低剂量组及维生素E组均可升高皮肤含水量和SOD活力,降低MDA含量,与模型组比较差异有显著性意义(P<0.01),并可改善皮肤组织的病理形态变化。【结论】精制蟒蛇油具有一定延缓皮肤衰老的作用,其机制与其能增加皮肤含水量和SOD活力,降低MDA含量有关。
Objective To study the effect of refined python oil on delaying D-galactose-induced skin aging in mice.Methods Kunming mice were randomized into high-,middle-and low-dose python oil groups（in the dosage of 10.0,5.0,and 2.5 mL/kg respecitively）,model group,normal group and positive control group（vitamin E,50mg for each mouse）.Except for the normal group,mice in the other groups were given daily subcutaneous injection of D-galactose（1g/kg） on the mice nape and back to induce sub-acute aging,and simultaneously were given skin application of the corresponding drugs for 42 continuous days.After treatment,the mice nape and back skin was sampled for the examination of water content,superoxide dismutase（SOD） activity and malondialdehyde（MDA） content.The skin histological features were also examined.Results In the model group water content and SOD activity were decreased,and MDA content was increased（P〈0.01 compared with those in the normal group）.Water content and SOD activity were increased,MDA content was decreased,and the pathological changes of the skin were improved in python oil groups and positive control group（P〈0.01 compared with those in the model group）.Conclusion Python oil exerts certain effect on delaying skin aging,and its mechanism is probably related with the increase of water content and SOD activity,and with the decrease of MDA content
出处
《广州中医药大学学报》
CAS
2011年第3期253-255,259,336,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
作者简介
任薇（1983-），女，硕士研究生
通讯作者：苏子仁（1964-），男，研究员；E-mail：suziren@gzhtcm．edu．cn
