摘要
应用扫描电子显微镜(SEM),傅立叶红外光谱(FTIR)和电子能谱(EDS)方法研究了酿酒酵母菌与铀酰离子的相互作用。电镜结果表明,吸附铀后酵母菌细胞发生一定变化,随时间延长细胞异常加剧;细胞表面有大量铀结晶,结晶量随铀浓度增加和作用时间延长而加大。能谱分析表明,酵母菌细胞表面结晶为含铀化合物。对照吸附前后的红外光谱图,细胞表面的O—H键作用后向低波数移动29cm-1,υs(—PO2-)、糖类的C—OH的伸缩振动均移动了约21cm-1,羧基(—COOH)的υC=O吸收带几乎完全消失;根据铀酰离子U—O键特征吸收峰推断形成的结晶可能为钙铀云母。结果表明,UO22+离子与酵母菌细胞表面发生了显著的吸附作用。。
The yeast （ Saccaromyces cerevisiae ） absorption of uranium was studied by using Scanning Electron Microscope （SEM）, Fourier Transform Infrared Spectrometry （FTIR） and Energy Dispersive X-ray Spectroscopy （EDXS）. The SEM results showed that yeast cell surface was covered with uranium crystal and the quantity of crystal increased with concentration of uranium and the interaction time. The surface morphology of yeast changed greatly with incubation time. EDXS analysis showed that the crystal of uranium was formed via adsorption on the surface of S. cerevisiae . FTIR results revealed that the peak attributed to υ s （O-H） of yeast cell surface shifted about 29 cm -1 to lower wavenumbers. Both peaks of υ s （C-OH） and υ s （-PO 2 - ） shifted about 21 cm -1 to lower wavenumbers, and the band attributed toυ C=O of the carboxyl group almost disappeared. The formed crystal is considered to be autunite according to the FTIR characteristic absorption band. These results indicated that the UO 2 2＋ can be stabilized via adsorption by S. cerevisiae .
出处
《高校地质学报》
CAS
CSCD
北大核心
2011年第1期53-58,共6页
Geological Journal of China Universities
基金
国家自然科学基金委员会-中国工程物理研究院联合基金资助项目《微生物对放射性核素的吸附富集研究》(10776027)
关键词
酿酒酵母菌
铀
吸附
表面作用
Yeast （ Saccaromyces cerevisiae ）
uranium
absorption
surface-interaction
作者简介
刘明学，1975年生，男，西南科技大学副教授，主要研究方向：矿物、核素一微生物相互作用；E-mail：dragonlmx@126．com
