摘要
从不同热应激强度(25,30,35,40℃)和40℃热应激后不同处理时间(0.5,1.5,2,2.5,4,6 h)两个角度,检测分析了獭兔血清中的丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)活力和总抗氧化能力(T-AOC)的大小,结果表明:与对照组25℃相比,30℃时獭兔血清的MDA略有下降(P>0.05);35℃和40℃时,血清MDA极显著降低(P<0.01)。獭兔血清的SOD活力和T-AOC分别呈升高和降低的趋势,各热应激组的SOD活力和T-AOC值与对照组相比,差异极显著(P<0.01)。在不同热应激时间处理下,獭兔血清的MDA先升高,在4 h时达到高峰(P<0.01),之后开始下降,在6 h时与0.5 h相比差异不显著。血清SOD活力和T-AOC值均处于上下波动状态,各热应激处理组的SOD活力均比0.5 h极显著升高(P<0.01)。血清的T-AOC值除在热应激处理1 h后显著升高(P<0.05)和1.5 h2、h后极显著下降外(P<0.01),其他各组无明显差异。
Rex rabbits were selected to analyze the changes of serum malondialdehyde（MDA）,total superoxide dismutase（T-SOD） and total antioxidant capacity（T-AOC） by using colorimetric method during heat stress with different intensity（25℃,30 ℃,35 ℃ and 40 ℃）and different time（0.5,1.5,2,2.5,4 and 6 hour）.The results showed as follows： Compared with the control group（25 ℃）,the MDA contents in serum decreased slightly（P0.05） after heat stress with 30 ℃ and it was significantly lower at 35 ℃ and 40 ℃（P0.01）.The SOD activity in serum of Rex Rabbit was in rising trend and T-AOC was in declining trend.The SOD activity and T-AOC-size in every heat stress group had very significant differences comparing with the control group.During heat stress with different time,the MDA contents in serum raised and reached the peak with 4 hour（P0.01）and then decreased.There were no differences between heat stress with 6 hour and 0.5 hour.The SOD activity and T-AOC-size in serum of Rex rabbit were in fluctuation.Compared with heat stress 0.5 hour,the SOD activity and T-AOC-size in every heat stress group were very significantly increased（P0.01）.The T-AOC-size in serum of Rex rabbit was significantly increased after heat stress with 1 hour（P0.05）and very significantly decreased after heat stress with 1.5 hour,2 hour（P0.01）.There were not differences between other groups for T-AOC-contents in serum of Rex Rabbit.
出处
《经济动物学报》
CAS
2011年第1期18-21,共4页
Journal of Economic Animal
基金
福建省科技厅科技项目资助(2008SZ0001-10)
关键词
獭兔
抗氧化指标
热应激
Rex rabbit
anti oxidation indices
heat stress
作者简介
刘庆华（1970-），男，副教授，主要从事草食动物遗传育种研究。通讯作者：刘庆华，E-mail：fauliu@126．com
