摘要
分离患"白点病"的大黄鱼(Pseudosciaena crocea)鱼鳃上的刺激隐核虫(Cryptocaryonirritans),提取基因组DNA,PCR扩增rDNA-ITS-1基因并进行序列分析.结果显示:该虫的rDNA-ITS-1序列与刺激隐核虫PYH4.12株及Chiayi虫株完全一致,表明福建霞浦流行的"白点病"病原为刺激隐核虫PYH4.12株或Chiayi株.以褐菖鮋(Sebastiscus marmoratus)为宿主,建立了该虫株的传代体系,对其生活史进行了详尽的观察,并收集各期虫体,为构建基因文库、功能基因的鉴定和分析奠定基础.
A strain of Cryptocaryon irritans was isolated from cultured Pseudosciaena crocea in Xiapu,Fujian,maintained and passaged successfully in aquaria by using Sebastiscus marmoratus as a host.The life cycle and the morphological characters of each phase were observed.Meanwhile,its genomic DNA was extracted and used as a template DNA to amplify the gene fragment of internal transcribed spacers of ribosomal DNA（rDNA-ITS-1） by using PCR.The sequence of rDNA-ITS1 was then analyzed and aligned with the known homological sequences of various strains of Cryptocaryon irritans.The result showed that both the morphological characterization and the sequence of rDNA-ITS-1 were identical to those of PYH4.12 and Chiayi strain,which inferred that the pathogen of ＇white spot disease＇ endemic in cultured Pseudosciaena crocea in Xiapu,Fujian is PYH4.12 or Chiayi strain of Cryptocaryon irritans.It provided with large numbers of the parasites for establishment of cDNA libraries and analysis of function genes.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第2期101-108,共8页
Journal of Fujian Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31040084)
福建省自然科学基金重点项目(2008J0004)
福建师范大学人才建设项目
关键词
刺激隐核虫
褐菖鲉
鉴定
传代
生活史
Cryptocaryon irritans
Sebastiscus marmoratus
identification
passage
life cycle
作者简介
通讯作者：黄晓红（1963-），研究员，博士，主要从事病原分子生物学研究．biohxh@fjnu．edu．cn
