摘要
目的探讨骨桥蛋白(osteopontin,OPN)和结缔组织生长因子(connective tissue growth factor,CTGF)及其联合检测在判断胃癌患者预后中的意义。方法应用免疫组化PowerVision法检测108例胃癌组织中OPN及CTGF的表达,分析OPN、CTGF表达与患者临床病理特征、术后生存时间之间的关系。结果 TNM III/IV期患者中OPN阳性率高于Ⅰ/Ⅱ期(P<0.001),OPN阳性的患者更易发生淋巴结转移(P=0.002)和远处转移(P=0.022),并且OPN表达与肿瘤浸润深度正相关(P=0.015)。CTGF高表达常见于发生淋巴结转移的病例(P=0.038)。OPN阳性的患者术后5年生存率低于OPN阴性的患者(P<0.001)。高表达CTGF的患者术后5年生存率低于低表达CTGF的患者(P=0.003)。分析联合检测OPN和CTGF在胃癌组织中的表达发现OPN阳性/CTGF高表达组、OPN阳性/CTGF低表达或者OPN阴性/CTGF高表达组与OPN阴性/CTGF低表达组3组之间患者的术后生存率存在显著差异(4.2%3、4.1%6、0.5%,P<0.001)。Cox回归分析结果表明TNM分期(P<0.001)、分化程度(P=0.01)和OPN/CTGF联合表达水平(P<0.001)是胃癌患者的独立预后因子。结论组织中OPN、CT-GF表达与胃癌的生物学特征密切相关,尤其是联合检测OPN/CTGF两种蛋白是指示胃癌患者预后的独立指标。
Purpose To detect the expression of osteopontin（OPN）and connective tissue growth factor（CTGF）in gastric carcinoma（GC）,and to investigate their prognostic significances.Methods Immunohistochemical method was used to detect the expression of OPN and CTGF in 108 cases of gastric carcinoma tissue samples.Results OPN was more frequently expressed in stages Ⅲ and Ⅳ GC than in stages Ⅰ and Ⅱ（P0.001）.Patients with OPN positive expression showed high incidence of lymph node metastasis（P=0.002）and distant metastasis（P=0.022）.OPN expression was closely correlated with deep invasion in the gastric wall（P=0.015）.High CTGF expression was significantly associated with lymph nodes metastasis（P=0.038）.High CTGF expression,OPN＋ and OPN＋/high CTGF expression were significantly associated with lower rates of postoperative 5-year overall survival（P0.001,P=0.003,P0.001,respectively）.Using a Cox regression analysis for the 108 patients,TNM stage（P0.001）,differentiation（P=0.01）and OPN/CTGF combined assay（P0.001）were independent prognostic indicators.Conclusions The expression of OPN and CTGF in gastric carcinoma was closely correlated with biological characteristics of GC.Combined analysis of OPN and CTGF has significant values in predicting the prognosis of gastric carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第2期130-134,共5页
Chinese Journal of Clinical and Experimental Pathology
关键词
胃肿瘤
骨桥蛋白
结缔组织生长因子
淋巴结转移
预后
stomach neoplasms
osteopontin
connective tissue growth factor
lymph node metastasis
prognosis
作者简介
刘鲁英，女，博士，副教授。Tel：（0543）3258654，E-mail：llixq@126．com
吴淑华，女，教授，通讯作者。Tel：（0543）3258654，E-mail：wsh6108@126．com
