摘要
目的利用高效液相色谱法测定太白贝母中的生物碱成分。方法采用Agilent Extend C18柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.02%三乙胺水溶液,梯度洗脱,流速为1.0 mL.min^-1,漂移管温度90℃;载气体积流量2.3 L.min-1,增益值2,进样量10μL;柱温25℃。结果贝母辛在28.75-690.0μg.mL^-1,贝母素乙在27.37-560.6μg.mL^-1,贝母素甲在25.75-527.0μg.mL^-1与峰面积的对数值呈线性关系,平均回收率分别为99.4%（n=6）,RSD为1.10%;99.7%（n=6）,RSD为3.61%;100.3%（n=6）,RSD为2.82%。结论太白贝母中的生物碱成分与川贝母非常相似,为其作为川贝母来源的合理性奠定了基础。
OBJECTIVE To develop a HPLC method for determining the contents of peimisine,imperialine and peiminine in Fritillaria taipaiensis.METHODS The analyses were performed on an Agilent Extend-C18 column（4.6 mm×250 mm,5 μm）.The investigated compounds were separated with gradient mobile phase consisting of methanol-water-triethylamine at the flow of 1.0 mL·min-1.The temperature for the detector drift tube was set at 90 ℃ and the nitrogen flow-rate was 2.3 L·min-1,the gain in ELSD was set at 2 in order to obtain the best sensitivity,and the injection volume was 10 μL.RESULTS The investigated compounds including peimisine,peiminine and peimine had good linearity（r2≥0.9991） within the tested ranges.The average recoveries were 99.4%,99.7% and 100.3% with RSD≤3.61%.CONCLUSION The developed method can be applied to control the quality of the F.taipaiensis with a good accuracy,sensitivity and repeatability.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第5期382-384,共3页
Chinese Pharmaceutical Journal
基金
科技部十一五支撑计划项目(2006BAI09B05-3)
中医药行业专项基金(200707007)
中医药行业专项基金(200807020)
作者简介
作者简介：段宝忠，男，硕士研究方向：中药资源与质量控制
通讯作者：黄林芳，女，博士，副研究员，硕士生导师研究方向：中药资源与质量控制Tel：（010）62811448E-mail：Ifhuang@implad．ac．cn
