摘要
在pH 4.39的乙酸钠-盐酸缓冲溶液中,盐酸环丙沙星与刚果红发生离子缔合反应,其最大吸收波长位于500nm处,表观摩尔吸光系数ε=9.46×104L/(mol.cm),盐酸环丙沙星浓度在0.8—3.2mg/L范围内呈良好的线性关系,线性回归方程为A=0.3635+0.01113C(mg/L),相关系数r=0.9966;检出限为9ng/L;RSD为1.6%,据此建立了测定盐酸环丙沙星的新方法。优化了对盐酸环丙沙星的测定条件。本法仪器设备简单,操作简便,体系稳定,回收率测定结果较满意。
A simple and rapid spectrophotometric method for the determination of ciprofloxacin hydrochloride was developed based on the interaction between congo red and ciprofloxacin hydrochloride in the buffer solution of NaAc-HCl for pH of 4.39.Under the optimal conditions of determination,there were good linear relationship in the range of 0.8—3.2mg/L for ciprofloxacin hydrochloride at the maximum absorption wavelength of 500nm,with ε of 9.46×104L/（mol·cm）.The equation of linear regression was A=0.3635＋0.01113C（mg/L,r=0.9966）,and the limit of detection was 9ng/L,while RSD was 1.6%.This method is applied to the analysis of ciprofloxacin hydrochloride samples with the simple instrument,convenient operates and the better recovery.
出处
《光谱实验室》
CAS
CSCD
北大核心
2011年第2期732-734,共3页
Chinese Journal of Spectroscopy Laboratory
基金
平顶山学院高层次人才科研启动经费资助项目(No.2008009)
关键词
分光光度法
盐酸环丙沙星
刚果红
Spectrophotometry
Ciprofloxacin Hydrochloride
Congo Red
作者简介
联系人。电话：（0375）4910185；传真：（0375）4910185；手机：（O）13782424267；E—mail：zhpan80@163．com作者简介：潘自红（1980-），女，河南省信阳市人，讲师，硕士，主要从事蛋白质及药物分析工作。
