摘要
目的评价口腔综合治疗台独立水源水路经次氯酸钠消毒前、后生物膜结构和菌落数情况。方法选择10台独立水源口腔综合治疗台,采集经525 mg/L次氯酸钠消毒水路前、后治疗台工作端水管内壁生物膜,计算总菌落数,并应用激光共聚焦扫描显微镜和扫描电镜观察生物膜结构。结果口腔综合治疗台水路消毒前、后生物膜菌落数几何均值分别为1.7×10~3 CFU/cm^2和OCFU/cm^2,两者比较,差异有显著性(t=12.03,P=0.02)。激光共聚焦扫描显微镜及扫描电镜观察结果显示,消毒前口腔综合治疗台水路内壁存在生物膜,呈特征性结构,杆菌与球菌分布于基质中;消毒后,其生物膜结构受到一定程度破坏,但基质仍然存在。结论口腔综合治疗台水路存在微生物污染,其内壁有生物膜,并含有高浓度细菌,存在引起医患医院感染的潜在风险。525 mg/L的次氯酸钠消毒剂对口腔综合治疗台水路具有较好的消毒效果,可常规应用于临床独立水源口腔综合治疗台水路的消毒处理。
Objective To evaluate the difference in the configuration and quantity of biofilms in dental unit water- lines （DUWI.） before and after disinfected with sodium hypochlorite solution. Methods The samples of biofilms from DUWI. supplied with 10 separate water sources were collected and the microorganisms were quantified after incubated for 48h, and the configuration of biofilms were observed with confoeal laser scanning microscope （CLSM） and scanning electronic microscope （SEM）. The sodium hypochlorite at concentration of 525 mg/L was used for disinfection of DUWL. Results The number of bacteria in biofilms at the baseline was 1.7 ×10^3CFU/cm^2 before disinfection and 0 CFU/cm^2 after disinfection with sodium hypochlorite solution, there was significant difference between the two （t = 12.03,P= 0.02） . CLSM and SEM showed that before disinfection, biofilm existed in the inner wall of DUWL, and bacilli and cocci distributed in the matrix; after disinfection, the configuration of biofilms, particularly fence-like structures were destroyed, but matrix was still existed. Conelusion DUWL has been contaminated heavily with a number of microbes, and the inner walls of DUWL is covered with biofilm, which is composed of millions of bacteria. The high concentration of microbes in DUWL is bringing the high risk of cross infection to both dental professionals and patients. Sodium hypoehlorite at the concentration of 525 mg/L may be used as a disinfec tion solution for DUWI. with separate water source.
出处
《中国感染控制杂志》
CAS
2011年第1期9-14,35,共7页
Chinese Journal of Infection Control
基金
广东省医学科研基金项目(A2008229)
关键词
口腔综合治疗台水路
生物膜
条件致病菌
次氯酸钠
消毒
感染控制
医院感染
dental unit waterlines
biofilm
opportunislic pathogen
sodium hypochlorite
disinfection
infection control
nosocomial infection
作者简介
章小缓（1966-），女（汉族），湖北省武汉市人，副主任医师，主要从事口腔内科学及医院感染管理研究。
[通讯作者]凌均棨E-mail：lingjq@mail．sysu．edu．cn
