摘要
研究了木质纤维原料预处理产生的主要碳水化合物降解产物对酿酒酵母乙醇发酵的影响,以及酿酒酵母对玉米秸秆酶水解液的乙醇发酵。碳水化合物降解产物对酿酒酵母NLH13乙醇发酵毒性依次为:甲酸>乙酸>糠醛>羟甲基糠醛,酿酒酵母NLH13乙醇发酵可耐受的甲酸和乙酸质量浓度分别为1和4 g/L,酿酒酵母NLH13在2～10 g/L范围内对糠醛和羟甲基糠醛有较强的耐受力。葡萄糖浓度为184.90 g/L的玉米秸秆酶水解液经酿酒酵母NLH13发酵24 h,糖利用率98.30%、乙醇质量浓度81.95 g/L和乙醇得率为88.41%。
Effects of degradation products of carbohydrates on the fermentation of Saccharomyces cerevisiae and the fermentation of corn stover enzymatic hydrolysate were investigated. The inhibition strengths of the typical inhibitors to ethanol fermentation were in the order of： formic acid 〉 acetic acid 〉 furfural 〉 hydroxymethylfurfural （HMF）. For ethanol fermentation, S. cerevisiae NLH13 could tolerate 1 g/L formic acid and 4 g/L acetic acid, respectively. However, S. cerevisiae NLH13 displayed higher tolerance to furfural and hydroxymethylfurfural, when their concentration in hydrolysate was 2-10 g/L. The sugar consumption, ethanol mass concentration and ethanol yield were 98.30 %, 81.95 g/L and 88.41%, respectively, when the corn stover enzymatic hydrolysate containing 184.90 g/L glucose was fermented with Saccharomyces cerevisiae NLH13 for 24 h.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2011年第1期9-12,共4页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(30871992)
国家863计划资助(2008AA05Z401)
林业公益性行业科研专项资助(201004001)
关键词
碳水化合物降解产物
发酵抑制物
乙醇
蒸汽爆破
degradation products of carbohydrates
fermentation inhibitor
ethanol
steam explosion
作者简介
宋晓川（1983-），女，吉林长春人，硕士，主要从事生物质资源生物转化的研究
通讯作者：勇强，教授，博士生导师，主要从事生物质资源生物降解与转化的研究；E-mail：swhx@njfu．com．cn。
