摘要
目的观察搜风祛痰法对血管紧张素Ⅱ致体外培养人脐静脉内皮细胞(HUVECs)iNOS及ET-1 mRNA表达的影响。方法制备兔活心汤含药血清。采用酶消化法培养HUVECs2-3代用于实验,采用RT-PCR法测定内皮细胞iNOSmRNA及ET-1 mRNA。结果活心汤可抑制AngⅡ导致的内皮细胞损伤,减少iNOSmRNA及ET-1 mRNA的表达。结论活心汤可抑制AngⅡ所致的HUVECs损伤,保护血管内皮细胞功能。
Objective：To observe the effects of expelling wind to eliminate phlegm method on the expression of human umbilical vein endothelial cells（HUVECs）iNOSmRNA and ET-1 mRNA in vitro induced by angiotensinⅡ.Methods：The serum containing metabolic ingredients of Huoxin Decoction was taken from rabbits.HUVECs2-3 were cultured in vitro by collagenase digestive method and iNOSmRNA and ET-1 mRNA were identified with RT-PCR method.HUVECs were randomly divided into 5 groups：the normal control group,the Ang Ⅱ group,the low concentration drug serum and Ang II group,the middle concentration drug serum and Ang Ⅱ group and the high concentration drug serum and Ang II group.Results：Huoxin Decoction could inhibit the injury of endothelial cells and decrease the expression of iNOSmRNA and ET-1mRNA.Conclusion：Huoxin Decoction can inhibit the injury of HUVECs induced by AngiotensinⅡto protect vascular endothelial.
出处
《中国中医急症》
2011年第2期259-261,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
辽宁省沈阳市科技基金项目(1071215)
作者简介
通信作者
