摘要
目的：用薄层荧光扫描法测定吴茱萸及加味左金丸中５种生物碱：羟基吴茱萸碱（ｈｙｄｒｏｘｙｅｖｏｄｉａｍｉｎｅ，Ｉ）、Ｎ甲酰二氢吴茱萸次碱（Ｎｆｏｒｍｙｌｄｉｈｙｄｒｏｒｕｔａｅｃａｒｐｉｎｅ，Ｉ）、吴茱萸酰胺甲（ｇｏｓｈｕｙｕａｍｉｄｅＩ，ＩＩ）、吴茱萸碱（ｅｖｏｄｉａｍｉｎｅ，ＩＶ）及吴茱萸次碱（ｒｕｔａｅｃａｒｐｉｎｅ，Ｖ）。方法：在高效硅胶薄层板上点样后，二次展开。展开剂：（１）氯仿—石油醚—甲醇—环己烷（５∶３∶０４∶０４）；（２）氯仿—甲醇（４１∶０３）。展开后用浓盐酸衍生化，荧光扫描测定。结果：各生物碱的线性范围在２０５～２４５ｎｇ，回收率为９６８％～１０４５％，并用此法测定了不同产地吴茱萸及不同批号的加味左金丸中生物碱的含量。结论：方法灵敏度高、样品用量少，可为寻找和利用植物资源，控制生药及方剂质量提供科学依据。
AIM: To adopt a TLC fluorescent
scanning method for the separation and determination of the alkaloids: hydroxyevodiamine (I),
Nformyldihydrorutaecarpine (II), goshuyuamideI (III), evodiamine (IV) and rutaecarpine (V) in
Evodia rutaecarpa and its preparation. METHODS: Chloroformpetroleum
ethermethanolcyclohexane (530404) was used as developing solvent on the HPTLC silica gel
plate for first development; and chloroformmethanol (4103) for the second development. After
that, the spots were derivatized by concentrated hydrochloric acid and then determined with a
Shimadzu CS930 TLC scanner. RESULTS: The calibration curves were linear in the range of
205245 ng for the five alkaloids and the recoveries were 968%1045%. The method has been
applied to the analysis of various samples and their preparations. CONCLUSION: This highly
sensitive method required small amount of sample. It offered a scientific basis for seeking and
utilizing botanic resources, and controlling the quality of herb medicines and preparations.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第5期383-386,共4页
Acta Pharmaceutica Sinica
关键词
薄层荧光扫描法
吴茱萸
生物碱
中药
thin layer chromatography fluorescent scanning
Evodia rutaecarpa
alkaloids
