摘要
目的评价ＭＴＴ甲萘醌增强法在眼科丝状真菌药物敏感性试验中的应用。方法用ＭＴＴ甲萘醌增强法测定二性霉素Ｂ和酮康唑对４株眼科丝状真菌（茄病镰刀菌、串珠镰刀菌、烟曲霉菌和黄曲霉菌）的最低抑菌浓度（ＭＩＣ）并与常量液体培养基稀释法（ＭａＡＦＳＴ）进行比较。结果在丝状真菌的药敏试验中，甲萘醌能明显增强ＭＴＴ的显色反应（镰刀菌加甲萘醌０．１ｍｍｏｌ／Ｌ，曲霉菌加１ｍｍｏｌ／Ｌ）。二性霉素Ｂ和酮康唑对丝状真菌有明显抑制作用，ＭＴＴ甲萘醌法所测定的Ａ值，随药物浓度的增加而显著降低，按Ｊａｈｎ氏等标准所测定的两种药物ＭＩＣ值与ＭａＡＦＳＴ测定的ＭＩＣ值接近。结论ＭＴＴ甲萘醌增强法在丝状真菌药物敏感试验中具有方便、快捷、灵敏的特点，值得进一步研究推广。
ObjectiveToevaluatemenadioneaugmentedMTTassayintheapplicationofantifungalsusceptibilityofocularfilamentousfungi.MethodsTheminimuminhibitoryconcentration(MIC)ofamphotericinBandketoconazoleto4strainsofocularclinicalisolatedfilamentousfungi(Aspergillusfumigatus,Aspergillusflavus,FusariumsolaniandFusariummoniliforme)weredeterminedbymenadioneaugmentedMTTassay.TheseMICvalueswerecomparedwithmacrodilutionantifungalsusceptibilitytest(MaAFST).ResultsThecolorationalreactionofMTTwasenhancedsignificantlybymenadione(0.11mmol/L)inthesusceptibilitytestingoffilamentousfungi.TheresultsshowedthatthefilamentousfungiwasinhibitedsignificantlyinthepresenceofamphotericinBorketoconazole,theabsorbency(A)valuesofmenadioneaugmentedMTTassaywasdecreasedfollowingtheincreaseoftheconcentrationofantifungaldrug.TheMICofamphotericinBandketoconazoleweredeterminedbymenadioneaugmentedMTTassay,correlatedexcellentlywithMaAFST.ConclusionThemenadioneaugmentedMTTassayisconvenientandeffectiveintheevaluationofantifungaleffectinvitro.
出处
《眼科研究》
CSCD
1999年第3期168-171,共4页
Chinese Ophthalmic Research
关键词
丝状真菌
眼部感染
药敏试验
MTT法
susceptibilitytestingfilamentousfungimenadioneaugmentedMTTassay
