摘要
目的研究冬凌草甲素(ORI)对膀胱癌EJ细胞株的抑制作用及机制。方法 MTT法检测ORI对EJ细胞的生长抑制作用;电子显微镜和Hoechst 33258染色法观察细胞的形态学变化;Western blot检测Bcl-2和Bax表达的变化。TRAP-PCR-银染法检测端粒酶活性的变化。结果 ORI对EJ细胞有明显的生长抑制作用,呈明显的时间-效应关系和浓度-效应关系。随着ORI作用时间延长,Bcl-2的表达逐渐减弱,Bax的表达逐渐增强。EJ细胞的端粒酶活性随着ORI作用时间的延长而逐渐降低。结论 ORI对膀胱癌EJ细胞有明显的生长抑制作用;机制可能是通过下调Bcl-2/Bax和通过抑制端粒酶活性诱发EJ细胞凋亡的。
Objective To study the antineoplastic effect of oridonin on bladder cancer cell lines,EJ cells in vitro.Methods The inhibitory rate of the cells was measured by MTT assay.Morphology of cell apoptosis was observed by Hoechst 33258 fluorescence staining and electron microscopy.Bcl-2 and bax expressions were detected by Western Blot.Telemerase activity was detected by telemerase repeat amplification proctocol（TRAP）-polymerase chain reaction（PCR）-silver stainning.Results Oridonin inhibited the growth of EJ cells and caused apoptosis significantly.The suppression was in a time-and dose-dependent manner.Oridonin down-regulated the bcl-2 expression and up-regulated the bax expression of EJ cells,decreased the activity of telemerase with time.Conclusion Oridonin has apparent inhibition and apoptosis-inducing effect on EJ cells,which may induce apoptosis via down-regulating Bcl-2/Bax and decreasing activity of telemerase in EJ cells.
出处
《现代泌尿外科杂志》
CAS
2011年第1期47-49,60,共4页
Journal of Modern Urology
基金
海南省卫生厅基金资助(No.200842)
作者简介
车宪平（1967-），男（汉族），博士，副主任医师．研究方向：泌尿系肿瘤．E—mail：chexianping@126．com
