摘要
目的:探讨法舒地尔对血小板源性生长因子(platelet-derived growth factor,PDGF)诱导的人肺动脉平滑肌细胞(human pulmonary artery smooth muscle cell,HPASMC)增殖的抑制作用及机制。方法:以体外培养的HPASMC为研究对象,PDGF-BB诱导增殖,法舒地尔进行预处理,MTT法检测细胞增殖;流式细胞仪检测细胞周期;Western blot检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和p27kip1蛋白的表达。结果:细胞培养24 h后与空白组比较,PDGF组HPASMC细胞增殖比例,S期细胞比例和PCNA蛋白表达明显增加,p27kip1蛋白表达则明显降低;用法舒地尔预处理后,与PDGF组比较,细胞增殖比例,S期细胞比例和PCNA蛋白表达明显下降,p27kip1蛋白表达则明显增加。结论:法舒地尔可通过上调p27kip1蛋白表达来抑制PDGF诱导的HPASMC增殖和细胞周期进程。
Objective To investigate the inhibiting effect of Fasudil on platelet-derived growth factor-induced human pulmonary artery smooth muscle cell proliferation and its mechanism.Methods Human pulmonary artery smooth muscle cells were cultured with the stimulation of platelet-derived growth factor-BB,and Fasudil in different concentrations was added before the addition of mitogen.Cell number and cell viability were determined with a hemocytometer and MTT assay respectively.The expressions of PCNA and p27kip1 protein were measured with Western blot analysis.Results Compared with control group,platelet-derived growth factor markedly induced human pulmonary artery smooth muscle cell proliferation,increased the percentage of cells in S phase and PCNA expression,but deceased p27kip1 expression.Pretreatment with Fasudil,however,significantly reversed the above effect induced by platelet-derived growth factor.Conclusion Fasudil can effectively inhibit the platelet-derived growth factor-induced human pulmonary artery smooth muscle cell proliferation and cell-cycle progression by up-regulating p27kip1.
出处
《中华实用诊断与治疗杂志》
2011年第1期7-10,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
国家自然科学基金项目(30972958)
作者简介
刘爱军（1976年-），男，博士，主治医师，研究方向：小儿心脏外科学。通讯作者：刘迎龙（1952年-），男，硕士，主任医师，教授，博士研究生导师，研究方向：小儿心脏外科学，E-mail：liucardio@163．com。
