摘要
【目的】探讨湖羊肌肉生长抑制素(myostain,MSTN)和生肌调节因子(myogenin,MyoG)基因表达的发育性变化及其与屠宰性状的相关性以及这两个基因表达水平的关联性。【方法】利用RT-PCR分析MSTN和MyoG基因在湖羊早期生长背最长肌肉组织中的mRNA的发育性变化。【结果】湖羊MSTN基因在肌肉中的表达在2日龄时表达水平最低,并随着日龄增加而增加直到60日龄为止,而后随着年龄增加而下降。湖羊MyoG基因在2日龄时表达最低,在30日龄之前随着日龄的增加而增加,然后在30日龄后下降,公羊直到120日龄、母羊直到90日龄为止,然后随着日龄的增加而又增加。MSTN和MyoG基因在湖羊各生长阶段间大多存在显著或极显著差异。湖羊公羊与母羊MSTN和MyoG基因之间在各相同生长阶段间大多存在显著或极显著差异。MSTN和MyoG基因表达水平与宰前活重、胴体重和净肉重均呈正相关,但只有MSTN和净肉重呈显著正相关(0.01<P<0.05),MSTN基因和MyoG基因的表达存在极显著正相关(P<0.01)。【结论】性别和年龄对于MSTN和MyoG基因在湖羊肌肉中的表达具有重要影响。MSTN、MyoG基因在不同生长阶段的公羊和母羊中的表达模式相似。MSTN和MyoG基因在湖羊不同生长阶段的肌肉中的表达均没有出现随着日龄的增加而一直增加或下降的趋势。MSTN基因和MyoG基因在湖羊早期肌肉性状中的表达为正相关。
【Objective】The objective of this study was to discuss developmental changes of gene expression of myostain （MSTN） and myogenin （MyoG） and their association analysis with carcass traits ,and the relationship of the expression level between MSTN gene and MyoG gene in Hu sheep.【Method】The developmental changes of gene expression of MSTN and MyoG on the early growth of Hu sheep were detected by RT-PCR analysis.【Result】MSTN gene expression in muscle of Hu sheep was the lowest at 2 days old,and tended to increase with the increasing age before 60 days,but after 60 days tended to decrease with increasing age.The MyoG gene expression in muscle of male sheep was the lowest at 2 days old,and tended to increase with the increasing age before 30 days,and after 30 days the expression first tended to decrease before 120 days（for male）or before 90 days （for female）,and then tended to increase with the increasing age.Among most of different stages there were significant differences or extreme significant difference for MSTN and MyoG gene in males and females of Hu sheep.There was a significant or extremely significant difference between male and female in most of different stages.The expression level of the MSTN and MyoG genes all showed a positive correlation with liveweight,carcass weight and net meat weight,but only MSTN showed a significant relationship with net meat weight （0.01P0.05）.MSTN gene showed an extremely significant positive relationship with MyoG（.P0.01）.【Conclusion】The age and gender played a very important role in the expression of sheep muscle.MSTN and MyoG genes showed a similar variation pattern for the male and female.Both of MSTN and MyoG gene expression in muscle of Hu sheep did not always tend to increase or decrease with the increasing age.MSTN gene showed an extremely significant positive relationship with MyoG gene on the early growth of Hu sheep.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第24期5129-5136,共8页
Scientia Agricultura Sinica
基金
中国博士后科学基金特别资助项目(200902154)
中国博士后科学基金(20080430470)
国家科技基础条件平台项目(2005DKA21101)
现代肉羊产业技术体系建设(MOA[2008]No.10)
江苏省自然科学基金(BK2007556)
"十一五"国家科技支撑计划子课题(2006BAD13B08-07)
江苏省苏北科技发展计划(BN2010004)
江苏省高校"青蓝工程"培养基金
苏州市农业科技支撑计划(SNG0911)
作者简介
作者简介：孙伟，副教授，博士。E—mail：dkxmsunwei@163．com
