摘要
目的研究百草枯（paraquat，PQ）中毒大鼠肺损伤时核因子（NF—κB）和肿瘤坏死因子（TNF）-α在肺组织中的表达，探讨PQ致肺损伤的机制。方法68只健康雄性Wistar大鼠随机分为对照组（8只）和染毒组（60只），以50mg／kgPQ一次性灌胃染毒，分别于1、3、7、14、28d处死，对照组予等体积生理盐水一次性灌胃，7d时处死，取血清和肺组织匀浆，用免疫组化链霉亲和素生物素标记法（LSAB）检测肺组织中NF—κBp65和TNF-α表达；同时测定血清、肺组织匀浆中丙二醛（MDA）含量及肺组织匀浆中羟脯氨酸（HPY）含量。结果染毒后1和3d时NF—κBp65[（20．33％±5．50％）、（27．50％±6．66％）1和TNF-α『（26．25％±8．50％）、（24．35％±8．51％）】有明显表达，与对照组[NF—κBp65（4．17％±2．48％），TNF—α（6．60％±3．97％）】比较，差异均有统计学意义（P〈0．05或P〈0．01），NF-κBp65和TNF-α分别表达于支气管上皮细胞和肺泡巨噬细胞。染毒组血清和肺组织匀浆中MDA含量明显增加，1、3、7d时血清中MDA含量【（10．15±3．15）、【6．97±1．65）、（5．44±0．66）nmol/ml]较对照组【（3．84±1．04）nmol/ml】明显增加，差异有统计学意义（P〈0．05或P〈0．01）；1、3d时肺组织匀浆中MDA含量【（10．20±2．43）、（10．71±171）nmol/ml】较对照组[（7．66±0．66）nmol/ml】明显增加，差异有统计学意义（P〈0．05或P〈0．01）。染毒组肺组织匀浆中HPY含量7d后逐渐增高，14、28d时[（19．98±2．86）、（26．06±4．06）μg／0．1g组织】明显高于对照组[（8．80±1．26）μg/0．1g组织】，差异有统计学意义（P〈0．01）。结论急性PQ中毒大鼠肺组织中NF—κB和TNF-α表达明显增加，NF—κB的活化可能参与PQ中毒肺损伤的发生。
Objective To investigate the expression of nuclear factor kappa B（NF-κB） and tumor necrosis factor α （TNF-α） in lung tissue of acute paraquat poisoned rats. Methods 68 male Wistar rats were randomly divided into 2 groups： the control group （n=8）, the intoxication group （n=60）. On the 1st, the 3rd, the 7th, the 14th and the 28th day after intoxication, the expression of NF-κB p65 and TNF-α in lung tissue were detected by LSAB immunohistochemistry（IH） staining. Meanwhile, the level of malondialdehyde（MDA） in plasma, and lung homogenate, the content of malondialdehyde （HPY） in lung homogenate were detected. Results The levels of MDA in plasma on the I st, the 3rd, the 7th day and in lung bomogenate on the 1 st, the 3rd day of the intoxication group[in plasma ：（ 10.15±3.15 ）, （6.97± 1.65 ） and （5.44±0.66） nmol/ml; in lung homogenate： （10.20±2.43）, （10.71 ±171 ） nmol/ml] were significantly higher than that of the control group [in plasma： （3.84±1.04） nmol/ml, in lung homogenate： （7.66±0.66） nmol/ml]. The content of HPY in lung homogenate on the 14th and the 28th day after intoxication [（19.98±2.86）, （26.06±4.06） μg/0.1 g lung homogenate] were higher than that of the control group[（8.80±1.26 ） μg/0. 1 g lung homogenatc] significantly. The expression of NF-κB p65 and TNF-α in lung tissue were both significantly increased on the first day and the 3rd day of the intoxication group compared with the control group and weakened obviously after the 7th day. Conclusion Acute paraquat poisoning can induce increased expression of both NF-κB p65 and TNF-α in lung tissue;the enhanced activity of NF-κB may take part in the process of puhnonary injury in PQ poisoning.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2011年第1期44-48,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
