摘要
平菇经热水抽提，乙醇沉淀，Ｓｅｖａｇ法去蛋白，乙醇分级分离，再经ＤＥＡＥ－纤维素柱层析得纯化多糖ＰＳＩ和ＰＳＩＩ，分别经聚丙烯酰胺凝胶电泳分析都呈一条带。在体外产生超氧阴离子自由基Ｏ２的反应体系中，平菇多糖组分ＰＳＩ和ＰＳＩＩ在较低浓度下（＜３００ｍｇ／Ｌ）都有清除Ｏ２的作用，而在较高浓度下（＞３００ｍｇ／Ｌ）作用不明显。
Polysaccharide PSI and PSII were isolated from the fruit bodies of Pleurotus ostreatus and subsequently purified by ethanol fractionation and DEAE-Cellulose chromatography;their homogineity were analysed by polyacrylamide gel eletrophoresis.The effects of PSI and PSII eliminating O_2 were also studied it indicated:PSI and PSII could eliminate O_2 in low concentration (<300mg/L)but their effects of eliminating O_2 were indistinctive in high concentration(>300mg/L).
出处
《食品科学》
EI
CAS
CSCD
北大核心
1999年第10期16-18,共3页
Food Science
