摘要
目的研究肺炎衣原体(chlamydia pneumoniae,Cpn)感染对高脂血症小鼠动脉粥样硬化形成的影响。方法 48只健康雄性C57BL/6J小鼠随机分为对照组、高脂组、感染组和感染+高脂组,每组12只。CPN感染于试验开始经鼻内每隔2周,共6次分别接种0.05 mL Cpn(6.6×106IFU/mL)。免疫荧光检测Cpn IgG抗体。初次感染后12周全部处死,放免法测定IL-6、IL-8的水平,取主动脉进行病理学检查。结果各组与对照组比较IL-6、IL-8的水平明显升高(P<0.05或P<0.01);且感染+高脂组与感染组相比差异有显著性(P<0.01)。感染组和高脂组无动脉粥样硬化的病理改变,感染+高脂组出现不同程度的动脉粥样硬化的变化。结论肺炎衣原体感染协同高脂血症诱导和/或加速动脉粥样硬化的形成。
【Objective】 To evaluate the effects of Chlamydia pneumonia infection on the development of artherosclerosis in C57BL/6J mice.【Methods】 Forty-eight 6 to 8 week old male C57BL/6J mice were fed with cholesterol-free standard chow diets（control and infected groups） or 1% cholesterol-supplemented chow（hyperlipidemia and infection hyperlipidemia groups）.The control and high lipidemia groups were inoculated with SPG buffer every two weeks,the infected and infected high lipidemia groups were inoculated with 6.6×106 inclusion-forming units of Cpn.Groups of mice were sacrificed at 12 weeks after the first inoculation.Lung tissues were tested for the presence of Cpn DNA,and radioimmunity assays demonstrated levels of IL-6 and IL-8.Histopathological changes of the aortic vessels were analyzed.【Results】 The levels of IL-6 and IL-8 significantly increased after infection with Chlamydia pneumonia in hyperlipidemia mice,in comparison with that in control group（P 0.05 or P 0.01）.Still the levels in infected hyperlipidemia group were higher than those in hyperlipidemia group（P 0.01）.There were different atherosclerotic plaques in infected hyperlipidemia group,no atherosclerotic plaques were seen in infected and hyperlipidemia groups.【Conclusion】 Chlamydia pneumonia infection could accelerate the process of atherosclerosis in hyperlipidemia mice.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第21期3236-3239,3244,共5页
China Journal of Modern Medicine
作者简介
[通信作者]李凡，E—mail：LiFan@jlu．edu.cn
