摘要
以羽毛角蛋白作为唯一碳源和氮源,从长期堆积腐烂羽毛的土壤中分离出一株高效降解羽毛角蛋白的菌株.通过对该菌株形态特征观察、生理生化实验测定、16S,rDNA序列分析和Biolog系统鉴定,初步鉴定为地衣芽孢杆菌(Bacillus licheniformis),且命名为地衣芽孢杆菌F4.F4在50,℃条件下摇瓶发酵48,h,角蛋白酶活达到23,U/mL.该菌能够降解完整的天然羽毛,具有开发应用前景.
The strain F4 from environment soil was isolated by feather meal as its sole source of carbon and nitrogen.The strain F4 was identified as Bacillus licheniformis based on morphology observation,physicochemical characteristics,16S rDNA sequence analysis and Biolog analysis.The keratinase activity reached 23 U/mL after 48,h of fermentation at 50,℃.The keratinases produced by this bacteria degraded natual feather without any treatment,and it had potential industrial application.
出处
《天津科技大学学报》
CAS
2010年第6期14-17,29,共5页
Journal of Tianjin University of Science & Technology
基金
国家科技基础条件平台课题(2005DKA21204-10)
关键词
角蛋白酶
地衣芽孢杆菌
筛选
鉴定
keratinase
Bacillus licheniformis
screening
identification
作者简介
李金婷（1984-），女，内蒙古兴安盟人，硕士研究生；
通信作者：路福平，教授，lfp@tust．edu．cn．
